Citotoksični učinak PCV 153 na proliferaciju CHO (Chinese Hamster Ovary)-K1 stanica

master's thesis

• Title (english): Cytotoxic effect of PCB 153 on CHO (Chinese Hamster Ovary)-K1 cell proliferation
• Author: Maja Vukmirović
• Mentor: Ivana Kmetič (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Toxicology); Zagreb
• Defense date and country: 2010-10-07, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U ovom radu ispitan je učinak PCB 153 na proliferaciju CHO (Chinese Hamster Ovary)-K1 stanica metodom Trypan Blue tijekom 72 sata. PCB 153 – jedan je od 209 spojeva (kongenera) iz skupine polikloriranih bifenila koji zbog svoje perzistentnosti i nakupljanja unutar prehrambenih lanaca predstavljaju opasnost za okoliš i članove ekosustava. Stanice su tretirane različitim koncentracijama PCB 153 (10 – 100 μM). Već nakon 24 sata, pri svim ispitanim koncentracijama PCB 153 osim kod najniže (10 μM), primijećeno je statistički značajno smanjenje broja stanica u odnosu na kontrolu. Tijekom dužeg perioda inkubacije (48 i 72 sata) inhibitorni učinak bio je i dalje izražen. Određene su IC vrijednosti nakon 24, 48 i 72 sata. IC50 vrijednost za PCB 153 nakon 72 sata inkubacije iznosila je 55,49 μM. Ispitivanje protektivnog učinka vitamina E na dinamiku rasta stanica tretiranih s PCB 153 ukazuje na poboljšanje rasta stanica međutim stanice su i dalje bile značajno inhibirane u odnosu na kontrolu.

Abstract (english)

In this study the cytotoxic effects of PCB 153 were examined by monitoring CHO (Chinese Hamster Ovary)-K1 cell proliferation using Trypan Blue method during 72 hours. PCB 153 is a congener from class of organic compounds known as polychlorinated biphenyls. Because of their persistance and accumulation within the food chains these compounds have adverse and long-lasting effects in environment. Cells were treated with different concentrations of PCB 153 (10 – 100 μM). After 24 hours all tested concentrations of PCB 153, apart from 10 μM, caused statistically significant decrease of cell proliferation compared to control. During longer period of incubation (48 and 72 hours) the inhibitory effect has been still expressed. IC values have been determined after 24, 48 and 72 hours. IC50 value for PCB 153 after 72 hours of incubation amounted 55.49 μM. Protective effect of vitamine E on proliferation of cells treated with PCB 153 was examined. Results showed an increase of cell proliferation, despite the fact that number of cells in group treated with PCB 153 together with vitamin E was far bellow the number of cells in control group.

• Keywords: PCB 153; CHO-K1 stanična linija; vitamin E; citotoksičnost; Trypan Blue metoda
• Keywords (english): PCB 153; CHO-K1 cell line; vitamin E; cytotoxicity; Trypan Blue
• Language: croatian
• URN:NBN: urn:nbn:hr:159:600344
• Study programme: Title: Food Technology; specializations in: Nutrition; Course: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer prehrambene tehnologije; smjer nutricionizam (diplomirani inženjer prehrambene tehnologije; smjer nutricionizam)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-08-28 09:01:44