Učinak različitih koncentracija glukoze, gutamina i piruvata na rast CCO stanica

master's thesis

• Title (english): Effects of various glucose, glutamine and pyruvate concentracion on CCO cell growth
• Author: Višnja Blajić
• Mentor: Igor Slivac (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2010-03-11, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Kultura životinjskih stanica je neizostavan dio suvremene biotehnologije. Metabolizam životinjskih stanica u kulturi pokazuje visoku deregulaciju u odnosu na metabolizam stanica u in vivo uvjetima. Karakteriziran je visokom i neučinkovitom potrošnjom glukoze i glutamina što dovodi do nakupljanja metaboličkih nusprodukata, laktata i amonijaka, u mediju koji inhibicijski djeluju na stanični rast. Cilj ovog rada bio je istražiti učinak glukoze i glutamina na rast CCO (Channel Catfish Ovary) stanica te pritom utvrditi količinu proizvedenog laktata i amonijaka. Također, istraživano je da li se piruvat može iskoristiti u zamjenu za glukozu i glutamin te time umanjiti nastajanje spomenutih nusprodukata. Istraživanje je pokazalo da početne koncentracije glukoze iznad 1 mM ne mijenjaju značajno brzinu rasta CCO stanica, dok se porastom početne koncentracije glutamina u mediju postiže brži rast stanica što dovodi do nakupljanja večih količina laktata i amonijaka. U mediju bez dodanog glutamina javlja se inhibicija rasta stanica. Piruvat u kompletnom mediju ne utječe značajno na brzinu rasta stanica te se povećanje njegove koncentracije ne može koristiti kao nadomjestak za glukozu ili glutamin i time umanjiti štetni učinak njihovih nusprodukata.

Abstract (english)

Animal cell culture is a very significant part of modern biotechnology. The metabolism of animal cells in culture shows high deregulation in relation to cell metabolism in in vivo conditions. It is characterised by the high and inefficient consumption of glucose and glutamine which leads to accumulation of lactate and ammonia in the culture media which have inhibition effect on the cell growth. The aim of this thesis was to investigate the effects of glucose and glutamine on the CCO (Channel Catfish Ovary) cell growth as well as the amount of produced lactate and ammonia. In addition, the effect of pyruvate as a substitute to glucose and glutamine was investigated. Results showed that the increase of glucose concentration in culture media does not lead to significant change of cell growth rate, while the increase of glutamine concentration achieves highest cell growth rate and thus formes a large quanity of ammonia and lactate. In culture media without added glutamine, cell growth inhibition occures. Pyruvate in complete culture media does not affect significantly on CCO cell growth rate, and its increased concentration can not be used as substitute for glucose or glutamine.

• Keywords: CCO; glukoza; glutamin; piruvat; metabolizam
• Keywords (english): CCO cells; glucose; glutamine; pyruvat; metabolism
• Language: croatian
• URN:NBN: urn:nbn:hr:159:601788
• Study programme: Title: Biotechnology; specializations in: Biochemical Engineering; Course: Biochemical Engineering; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo (diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-08-28 09:12:46