Citotoksični učinak PCB-77 na proliferaciju CHO-K1 stanica

master's thesis

• Title (english): Cytotoxic effect of PCB-77 on proliferation on CHO-K1 cells
• Author: Kristina Beljan
• Mentor: Ivana Kmetič (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Toxicology); Zagreb
• Defense date and country: 2010-07-16, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Poliklorirani bifenili su perzistentni organski zagađivači okoliša koji uzrokuju mnoga negativna djelovanja na živa bića. Cilj ovog istraživanja bio je odrediti djelovanje PCB-77 na proliferaciju CHO-K1 stanica. Za praćenje citotoksičkog učinka PCB-77 primijenjena je stanična linija CHO-K1 uzgojena u Dulbecco MEM mediju uz dodatak 10% FBS. Stanice su tretirane različitim koncentracijama PCB-77 (25, 50, 75 i 100 μM). Citotoksički učinak 25-100 μM PCB-77 određen je MTT metodom tijekom 24, 48 i 72 sata. Nakon 72 sata, inhibicija proliferacije iznosila je 34,7-79,3%. IC vrijednosti su izračunate iz jednadžbi interpoliranih eksponencijalnih krivulja s grafičkog prikaza ovisnosti % inhibicije proliferacije stanica pri korištenim koncentracijama PCB-77. IC50 vrijednost za PCB-77 nakon 72 sata iznosila je 43,1 μM. Dobiveni rezultati ukazuju na inhibitorni učinak PCB-77 ovisno o povećanju doze.

Abstract (english)

The polychlorinated biphenyls are persistent organic pollutants that cause many adverse effects on living beings. The objective of this research was to determine effects of PCB-77 on proliferation of CHO-K1 cell line. CHO-K1 cell line cultivated in Dulbecco's MEM medium suplemented with 10% FBS was applied as the test system for cytotoxicity determination of PCB-77. Test groups were treated with different concentrations of PCB-77 (25, 50, 75 and 100 μM). Cytotoxic effect was assessed by MTT method during 24, 48 and 72 h. After 72 h, dose respondent inhibition of proliferation amounted for 34.7-79.3%. IC values were derived from equations of related exponential trend lines interpolated in graphically presented correlation between % of cell growth inhibition and applied PCB-77 concentrations. IC50 for PCB-77 after 72 h was 43.1 μM. The results indicate that PCB-77 inhibits the proliferation of CHO-K1 cells. Inhibition of proliferation was proportional to increase of concentration of PCB-77.

• Keywords: CHO (Chinese Hamster Ovary)-K1 stanična linija; PCB-77; citotoksičnost; in vitro test; MTT metoda
• Keywords (english): CHO (Chinese Hamster Ovary)-K1 cell line; PCB-77; cytotoxicity; in vitro test; MTT method
• Language: croatian
• URN:NBN: urn:nbn:hr:159:784579
• Study programme: Title: Food Technology; specializations in: Nutrition; Course: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer prehrambene tehnologije; smjer nutricionizam (diplomirani inženjer prehrambene tehnologije; smjer nutricionizam)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-08-28 09:54:03