Utjecaj agroekoloških uvjeta uzgoja na biološki aktivne spojeve lista i korijena batata (Ipomoea batatas L.)

master's thesis

• Title (english): The influence of agroecological conditions on biologically active compounds in leaves and roots of sweet potato (Ipomoea batatas L.)
• Author: Tihana Kralj
• Mentor: Ivana Radojčić Redovniković (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2010-03-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Batat ima veliku nutritivnu vrijednost i ljekovita svojstva. Bogat je prehrambenim vlaknima, mineralima, vitaminima i antioksidansima. Listovi sadrže visoku koncentraciju biološki aktivnih polifenolnih spojeva. Polifenolni sastav te antioksidativni kapacitet batata razlikuje se između kultivara, ovisno o okolišnim i uzgojnim čimbenicima. U radu je određivan ukupni polifenolni sastav i antioksidacijski kapacitet uzoraka korijena i listova dviju sorti batata uzgojenih s različitim mineralnim gnojivima (omjeri dušika i kalija 1:1, 1:2,5 i 1:5), te dodatkom Zeolita i FitoLife-a. Za određivanje ukupnih polifenola primjenjena je spektrofotometrijska metoda s Folin-Ciocalteau reagensom. Antioksidacijski kapacitet uzoraka određivan je ORAC (Oxigen Radical Absorbance Capacity), ABTS [2,2´-azinobis(3-etilbenzotiazolin-6-sulfonat)] i FRAP (Ferric Reducing Antioxidant Power) metodama. Rezultati pokazuju da list batata sadrži više polifenolnih spojeva od korijena. Mineralna gnojiva te dodatak Zeolita i FitoLife-a imali su negativan utjecaj na ukupne polifenole u listovima kao i u korijenu Boniato sorte, dok se povećao udio u korijenu Bat1 sorte. Udjeli ukupnih polifenolnih spojeva su u linearnoj korelaciji s vrijednostima antioksidacijskog kapaciteta.

Abstract (english)

Sweet potato has great nutritional and health properties. They are rich in dietary fiber, mineral, vitamins and antioxidants. Sweetpotato leaf contains high concentrations of polyphenolics. Polyphenolic content and antioxidant activity in sweet potato differed between cultivars due to environmental and cultivation factors. We studied the total polyphenolic content and antioxidant activity of root and leaf samples of two sweet potato varieties grown with different fertilization (nitrogen-potassium ratios 1:1, 1:2,5 and 1:5) and Zeolit and FitoLife addition. Total polyphenolic content was determined by Folin-Cicalteu method, while antioxidant capacity was determined by ORAC (Oxigen Radical Absorbance Capacity), ABTS [2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid)] i FRAP (Ferric Reducing Antioxidant Power) assays. The results showed that sweet potato leaves contained more polyphenols from the roots. We found negatively influence of mineral fertilization, Zeolit and FitoLife addition on total polyphenolic content in leaves as well as for Boniato roots while in Bat1 root we found increase of total polyphenols. In addition, total polyphenolic content were highly correlated with values of antioxidant capacity determined by all three methods.

• Keywords: ABTS metoda; batat; FRAP metoda; ORAC metoda; polifenolni spojevi
• Keywords (english): ABTS assay; FRAP assay; ORAC assay; polyphenols; sweet potato
• Language: croatian
• URN:NBN: urn:nbn:hr:159:616496
• Study programme: Title: Food Technology; specializations in: Nutrition; Course: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer prehrambene tehnologije; smjer nutricionizam (diplomirani inženjer prehrambene tehnologije; smjer nutricionizam)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-08-28 12:19:19