Utjecaj djelovanja ultrazvuka visokog intenziteta na određivanje veličine čestica metodom laserske difrakcije

master's thesis

• Title (english): Effect of high intensity ultrasound on the determination of particle size by laser diffraction method
• Author: Tatjana Đunđuš
• Mentor: Mladen Brnĉić (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Process Engineering); (Laboratory for Unit Operations); Zagreb
• Defense date and country: 2010-10-12, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Rad je temeljen na ispitivanju metode određivanja raspodjele veličina čestica sulfizoksazol acetila laserskom difrakcijom, u svrhu osiguranja kvalitete. Ispitano je djelovanje ultrazvuka od 10 %, 25 %, 50 %, 75 % i 100 % pri brzinama miješanja od 2000 o/min i 2500 o/min. Snaga ultrazvuka 100 % iznosi 26 W, tako da je ispitano djelovanje pri 2.6 W, 6.5 W, 13 W, 19.5 W i 26 W. Provedena su mjerenja prije djelovanja ultrazvuka (nakon dodatka uzorka u kupelj disperzione jedinice Hydro 2000 S) i za vrijeme djelovanja ultrazvuka. Malvern Mastersizer instrumenti imaju sve potrebne opcije mjerenja i dijagnoze koji podupiru sve standarde dobre proizvođačke prakse. Validirana je standardna procedura analize raspodjele veličina čestica na instrumentu Malvern Mastersizer 2000. Pokazalo se da je ultrazvuk poboljšao ujednačenost raspodjele veličine čestica. Najbolji rezultati postignuti su kod nominalne snage ultrazvuka od 13 W tj. 50 %. Pokazalo se također kako povećanje snage ultrazvuka na 75 % učinkovito razbija aglomerate ali također djeluje i na razbijanje čestica. Dobivene vrijednosti eksperimentalnog rada su u skladu sa zahtjevima ISO 13320-1, te je validirana metoda laserske difrakcije (MS2000) kao pogodna metoda za rutinsko određivanje veličina čestica.

Abstract (english)

This work was based on examination of laser diffraction method for volume distribution of particle sizes sulfisoxazole acetyl, to establish safe quality control. We examined the effect of ultrasound of 10 %, 25 %, 50 %, 75 % and 100 % mixing at speeds of 2000 rpm and 2500 rpm. Ultrasound power at 100% is 26 W, so the effect was examined at 2.6 W, 6.5 W, 13 W, 19.5 W and 26 W. Measurements were made before the action of ultrasound (after addition of sample in dispersion units Hydro Bath 2000 S) and during effects of ultrasound. Malvern Mastersizer instruments have all the options of measurement and diagnosis of supporting all standards of good manufacturing practices. Validated the standard procedure of analysis of particle size distribution on the instrument Malvern Mastersizer 2000 It was found that ultrasound improved the uniformity of particle size distribution. The best results were achieved with nominal power of 13 W ultrasound at 50 %. It has been shown also to increase the power of ultrasound at 75 % effective smashing agglomerates but also affects the breaking of particles. The values obtained experimental work in accordance with the requirements of ISO 13320-1, and is validated method of laser diffraction (MS2000) as a suitable method for routine determination of particle size.

• Keywords: raspodjela veličina čestica; ultrazvuk; laserska difrakcija
• Keywords (english): distribution of particle sizes; ultrasound; laser diffraction
• Language: croatian
• URN:NBN: urn:nbn:hr:159:487624
• Study programme: Title: Biotechnology; specializations in: Biochemical Engineering; Course: Biochemical Engineering; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo (diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-08-29 07:22:38