Određivanje prisutnosti bakterije Listeria monocytogenes u svježjem kravljem siru imunoenzimskom metodom

master's thesis

• Title (english): Detection of Listeria monocytogenes in cow cheese immunoassay method
• Author: Mirna Klarić
• Mentor: Frane Delaš (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for General Microbiology and Food Microbiology); Zagreb
• Defense date and country: 2010-09, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Cilj ovog rada je bio imunoenzimskom metodom odrediti prisutnost bakterija roda Listeria spp. u uzorcima svježeg kravljeg sira, a u dobivenim pozitivnim uzrocima postupcima horizontalne metode za dokazivanje i određivanje broja stanica metodom kultivacije i biokemijski dokazati prisutnost vrste Listeria monocytogenes. Također trebalo je usporediti rezultate dobivene imunoenzimskom metodom i metodom kultivacije. Ispitivano je ukupno 30 nasumično izabranih uzoraka svježeg kravljeg sira dostupnih na zagrebačkim tržnicama. Dobiveni rezultati su uspoređeni s kriterijima navedenim u važećoj zakonskoj regulativi Republike Hrvatske. U jednom od 30 uzoraka je utvrđena prisutnost bakterije Listeria monocytogenes, a u tri uzorka je utvrđena prisutnost Listeria innocua.

Abstract (english)

The aim of this study was to determine the presence of Listeria spp. Immunoassay method in samples of fresh cow cheese, and from the obtained positive samples with Horizontal method for the detection and enumeration of cells and biochemical prove the presence of species Listeria monocytogenes. Also is need to compare the results obtained by Immunoassay method and by Horizontal method. It research involve a total of 30 randomly selected samples of fresh cow cheese that are available in Zagreb on markets. The results obtained are compared with the criteria set forth in the applicable legislation of the Republic of Croatian. In one of the 30 samples is showed presence of Listeria monocytogenes, and in three samples was determined by the presence of Listeria innocua.

• Keywords: imunoenzimska metoda; L. monocytogenes; mikrobiološka i zdravstvena ispravnost; svježi sir
• Keywords (english): fresh cheese; immunoassay method; L. monocytogenes; microbiologic quality
• Language: croatian
• URN:NBN: urn:nbn:hr:159:458366
• Study programme: Title: Biotechnology; specializations in: Biochemical-Microbiology; Course: Biochemical-Microbiology; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki (diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-08-29 08:08:39