S-proteini kao medijatori adhezijskih svojstava Lactobacillus paraplantarum SF9B

master's thesis

• Title (english): S-proteins mediate adhesion properties of the strain Lactobacillus paraplantarum SF9B
• Author: Martina Vešligaj
• Mentor: Blaženka Kos (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology); Zagreb
• Defense date and country: 2016-09-23, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Bakterijska adhezija na crijevni epitel i kolonizacija gastrointestinalnog trakta su vrlo važne odlike probiotičkih bakterija. S-proteini koji se nalaze na površini probiotičkih bakterija uvelike doprinose tom procesu. Cilj ovog rada bio je ispitati ulogu S-proteina kao medijatora adhezijskih svojstava soja Lactobacillus paraplantarum SF9B, pri čemu je kao pozitivna kontrola korišten soj Lactobacillus helveticus M92 koji eksprimira S-proteine, a kao negativna soj Lactobacillus plantarum D13 koji ne ekprimira S-proteine na svojoj površini. Osim S-proteina, na površini stanica soja L. paraplantarum SF9B nalaze se i proteini koji su na njih vezani (eng. SLAP - Surface-Layer Associated Proteins). Dokazano je da S-proteini soja L. paraplantarum SF9B pozitivno utječu na sposobnost adhezije na mucin, kao i na adheziju na stanice Caco-2 stanične linije. Stanice navedenog soja bile su pokazale uspješnost kompetitivne ekskluzije sa test-mikroorganizmima Salmonella enetrica serovar Typhimurium FP1 i Escherichia coli 3014. Turbidimetrijskim određivanjem brzine rasta u različitim uvjetima ustanovljeno je da soj L. paraplantarum SF9B bolje preživljava u nepovoljnim uvjetima u odnosu na soj L. plantarum D13. Na kraju, stanice soja L. paraplantarum SF9B pokazuju značajnu proteolitičku aktivnost.

Abstract (english)

Bacterial adhesion and colonization of the intestinal epithelium of the gastrointestinal tract are very important features of probiotic bacteria. S-layer proteins that are expressed on the surface of probiotic bacteria greatly contribute to this process. The aim of this study was to examine the S-layer proteins as mediators in adhesive properties of the strain Lactobacillus paraplantarum SF9B, while the strain Lactobacillus helveticus M92 which express S-layer proteins was used as a positive control, and the strain Lactobacillus plantarum D13 which does not express S-layer proteins was used as a negative control. In addition to S-layer proteins, on the cell surface of L. paraplantarum SF9B there are SurfaceLayer Associated Proteins (SLAPs). It has been shown that S-layer proteins of L. paraplantarum SF9B positively influence the adhesiveness to mucin and the adhesion to cells of the Caco-2 cell line. Cells of that strain demonstrated success in competitive exclusion of test-microorganisms Salmonella enterica serovar Typhimurium FP1 and Escherichia coli 3014. Turbidimetric determination of the rate of growth in different conditions found that the strain L. paraplantarum SF9B survived better in adverse conditions with respect to the strain L. plantarum D13. Finally, strain L. paraplantarum SF9B showed significant proteolytic activity.

• Keywords: S-proteini; SLAP; Lactobacillus paraplantarum; adhezija; kolonizacija
• Keywords (english): S-layer proteins; SLAP; Lactobacillus paraplantarum; adhesion; colonization
• Language: croatian
• URN:NBN: urn:nbn:hr:159:712062
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-24 12:21:31