Učinci seruma, laktata i amonijaka na rast CCO stanica

master's thesis

• Title (english): Serum, lactate and ammonia effects on CCO cell growth
• Author: Eva Cvitaš
• Mentor: Igor Slivac (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2009, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Kultura životinjskih stanica je važan dio biotehnologije te srodnih znanstvenih i proizvodnih područja. Njihova primjena je raznolika, od proučavanja stanične strukture, fiziologije i diferencijacije pa sve do proizvodnje mnogih biološki aktivnih proizvoda. U radu s kulturama životinjskih stanica provode se istraživanja koja ukazuju na najpovoljnije uvjete za njihov uzgoj i održavanje. Cilj ovog rada je utvrditi učinke nekih sastojaka hranjivog medija na karakteristike rasta CCO stanične linije. Istraživan je učinak fetalnog goveđeg seruma (FBS) koji se dodaje staničnoj kulturi zbog njegova proliferativnog djelovanja te učinci laktata i amonijaka koji su proizvodi staničnog metabolizma. Istraživanjem je ustanovljeno da količina seruma dodanog u hranjivi medij utječe na brzinu rasta stanica. Pri istraživanju učinka na stanični rast, uočeno je da je najveća brzina rasta stanica ostvarena u hranjivom mediju s 10% FBS-a, a najmanja u mediju s 1% FBS-a. Što je veća koncentracija amonijaka (NH4Cl) u mediju, to je njegov inhibirajući učinak izraženiji. U kulturi s oko 5 mM NH4Cl prestaje rast CCO stanica, a pri koncentracijama većim od 5 mM stanice odumiru. Laktat u kulturi CCO stanica također usporava stanični rast. Pri koncentraciji preko 7,5 mM u kulturi stanica zapaženo je njihovo odumiranje. Pojavu postupnog smanjivanja koncentracije laktata u hranjivom mediju moguće je objasniti djelovanjem unutarstaničnih laktat-dehidrogenaza oslobođenih iz citosola odumrlih stanica.

Abstract (english)

Animal cell culture is a significant part of modren biotechnology and many other scientific and production related fields. It is used in various studies, from cell structure, physiology and diferentiation researches to active biological components production. A purpose of cell culture investigation is to identify the most acceptable cell cultivation and maintenance condiotions. An object of this study is to establish the main CCO cell growth caratheristics giving special attention to the serum, lactate and ammonia growth effects. It is well known that certain concentrations of foetal bovine serum (FBS) stimulate cell proliferation while lactate and ammonia as metabolic products exert inhbitory effects. The amount of serum added to culture media showed various changes on CCO cell growth. During the research of FBS effect on cell growth, the highest cell growth rate is found in DMEM medium with 10% FBS and the lowest in medium with 1% FBS. The higher the concentration of NH4Cl in medium, the more expressive the inhibiting effect. CCO cell growth stops at approximately 5 mM NH4Cl in culture media. At even higher concentrations the cells begin to die. CCO cell growth is also inhibited by lactate. It was found that lactate concentrations higher than 7,5 mM have lethal effect on CCO cell lines. The decrease in the observed specific lactate production can be related to intracellular lactate dehydrogenase activity of dead cells.

• Keywords: amonijak; CCO; laktat; metabolizam; serum
• Keywords (english): ammonia; CCO; lactate; metabolism; serum
• Language: croatian
• URN:NBN: urn:nbn:hr:159:582412
• Study programme: Title: Biotechnology; specializations in: Biochemical Engineering; Course: Biochemical Engineering; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo (diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-08-30 10:09:13