Antimikrobno djelovanje konzervansa metilparabena u tekućem farmaceutskom pripravku

master's thesis

• Title (english): Antimicrobial efficacy of preservative methylparaben in liquid pharmaceutical
• Author: Iva Pavelić
• Mentor: Frane Delaš (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for General Microbiology and Food Microbiology); Zagreb
• Defense date and country: 2008-12-12, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Testom antimikrobnog djelovanja istraživan je antimikrobni učinak konzervansa metilparabena u oralnoj vodenoj suspenziji. Testiranju su podvrgnuti uzorak originalnog lijeka i tri uzorka generičkog lijeka različitih koncentracija konzervansa – ciljana koncentracija, 80%-tna i 50%-tna koncentracija od ciljane. Istraživanje se provodi namjernom kontaminacijom uzorka inokulumima propisanih test mikroorganizama, čuvanju kontaminiranog uzorka na propisanoj temperaturi, uzimanju uzorka u određenim vremenskim intervalima i određivanju broja test mikroorganizama. Sve upotrebljene koncentracije konzervansa metilparabena pokazale su se učinkovitim protiv upotrebljenih test mikroorganizama. Primjenom metode za određivanje mikrobiološke čistoće validirana je metoda za određivanje broja živih aerobnih mikroorganizama i stanica kvasca i plijesni korištena u testu antimikrobnog djelovanja. Na temelju svih dobivenih rezultata zaključuje se da je upotrebljeni uzorak konzervansa metilparabena pogodan za konzerviranje korištenog uzorka oralne vodene suspenzije.

Abstract (english)

Antimicrobial effectiveness test has been used for examination of the antimicrobial preservative methylparaben in oral aqueous suspension. The sample of the original drug and three samples of generic drug with different concentrations of preservative – target concentration, 80% and 50% concentration of target has been tested. The test consists of challenging the preparation, with prescribed inoculum of test microorganisms, storing the inoculated preparation at a prescribed temperature, withdrawing samples at specified intervals of time and counting the microorganisms. All used concentrations of the preservative methylparaben has been shown effective against the test microorganisms. Applying the method for microbiological examination, the counting method used in antimicrobial effectiveness testing, has been validated. On grounds of all given results the conclusion is that the used sample of preservative methylparaben is suitable for preservation of used sample oral aqueous suspension.

• Keywords: antimikrobno djelovanje; konzervans; metilparaben; test mikroorganizmi; validacija
• Keywords (english): antimicrobial efficacy; methylparaben; preservative; test microorganisms; validation
• Language: croatian
• URN:NBN: urn:nbn:hr:159:181286
• Study programme: Title: Biotechnology; specializations in: Biochemical-Microbiology; Course: Biochemical-Microbiology; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki (diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-08-31 09:50:50