The percentage of MRSA in nose swab samples taken from subjects in an elderly home in order to examin their sensitivity to antibiotics

master's thesis

• Title: Postotak MRSA izolata u brisevima vestibuluma nosa institucionaliziranih starijih korisnika i ispitivanje osjetljivosti na antibiotike
• Author: Danijela Radošević
• Mentor: Jagoda Šušković (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology); Zagreb
• Defense date and country: 2008-12-17, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U okviru praćenja postotka bakterije meticilin rezistentni Staphylococcus aureus u institucionaliziranih starijih korisnika jednog Zagrebačkog doma umirovljenika provedeno je ispitivanje antimikrobne osjetljivosti sojeva Staphylococcus aureus i određivanje prisutnosti mecA gena PCR metodom. Svrha ovog rada je na osnovu podataka dobivenih istraživanjem o prisutnosti MRSA pristupiti dekolonizaciji kako bi se spriječilo daljnje širenje. Prikupljeno je 26 sojeva testiranih na osjetljivost 12 antibiotika: penicilin, cefoksitin, oksacilin, klindamicin, azitromicin, gentamicin, ciprofloksacin, rifampicin, vankomicin, linezolid, fucidinsku kiselinu i mupirocin. Rezistencija na meticilin utvrđena je detekcijom mecA gena. Zaključak je ove analize da je prevalencija MRSA u Zagrebačkom domu umirovljenika 9,52%, a PCR analizom je utvrđena prisutnost mecA gena u svim izolatima.

Abstract (english)

The samples of MRSA from an elderly home in Zagreb were taken to investigate the sensitvity of these bacteria to antibiotics and to see if the bacteria contains the gene mecA using the PCR method. The aim of this experiment is, based on the results of survey, to identify the right antibiotics which will decolonise the bacteria and prevent further spreading of it to the healthy elders in the home. 26 samples were collected and tested for 12 different antiobiotics. The existance of the gene mecA on the bacteria's chromosome indicates a resistance to meticilin. The conclusion of this experiment is that 9,52% of the population of the elderly home have MRSA and the PCR method has shown that every sample of MRSA bacteria contains the macA gene.

• Keywords: MRSA; Staphylococcus aureus; mecA
• Keywords (english): MRSA; Staphylococcus aureus; mecA
• Language: croatian
• URN:NBN: urn:nbn:hr:159:821628
• Study programme: Title: Biotechnology; specializations in: Biochemical-Microbiology; Course: Biochemical-Microbiology; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki (diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-08-31 10:00:25