Validacija enzimno-imunološke metode za određivanje flumetazona i deksametazona u mlijeku i jetri životinja

master's thesis

• Title (english): Validation of the enzyme immunoassay method for measuring in the content of flumethasone and dexamethasone in animal's milk and liver
• Author: Nataša Piščević
• Mentor: Jasna Vorkapić-Furač (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2009-11-13, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U ovom radu validirana je enzimno-imunološka metoda (EIA), analitička metoda za određivanje sadržaja flumetazona i deksametazona u mlijeku i jetri životinja. Određeni su sljedeći parametri validacije: limit detekcije i kvantifikacije, sposobnost detekcije, iskorištenje metode, preciznost i unutarlaboratorijska ponovljivost metode te robusnost metode. Nakon provedenog postupka validacije zaključeno je da metoda zadovoljava sve postavljene kriterije te se smatra prikladnom za predviđenu i buduću primjenu.

Abstract (english)

In this thesis, the enzyme immunoassay method (EIA), an analytical method for measuring the contents of flumethasone and dexamethasone in animal’s milk and liver, has been validated. The following parameters of validation have been defined: the limit of detection and quantification, detection ability, method usage, precision and interlaboratory repeatability of the method and the robustness of the method. After the validation, it has been concluded that the method satisfies all the given criteria and is suitable for presupposed and future application.

• Keywords: EIA metoda; validacija metode; deksametazon; flumetazon
• Keywords (english): EIA; method validation; flumethasone; dexamethasone
• Language: croatian
• URN:NBN: urn:nbn:hr:159:243693
• Study programme: Title: Biotechnology; specializations in: Biochemical Engineering; Course: Biochemical Engineering; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo (diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-08-31 12:21:40