Revalidacija metode "Kvalitativno određivanje komponenata benzina i dizel goriva SPME-GC/MS metodom"

master's thesis

• Title (english): Revalidation of the method "Qualitative determination of gasoline and diesel fuel components using SPME-GC/MS method"
• Author: Maja Žunko
• Mentor: Veronika Kovač (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Organic Chemistry); Zagreb
• Defense date and country: 2009-10-06, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Napravljena je revalidacija metode koja se primjenjuje kod vještačenja lakozapaljivih tekućina (benzina i diesela). Metoda uklučuje upotrebu SPME vlakna za ekstrakciju uzoraka te GC/MS vezanog sustava za analizu. U sklopu revalidacije ispitani su parametri: SPLIT i SPLITLESS način rada plinskog kromatografa, vrijeme zagrijavanja uzorka, vrijeme uzorkovanja, brzina zagrijavanja kolone plinskog kromatografa, granica dokazivanja benzina i diesela (LOD) te utjecaj matriksa. Identificirani su organski spjevi prisutni u benzinu i dieselu, a prilikom identifikacije poštivani su kriteriji S/N≥3, QM≥70, prisutnost karakterisičnih ionskih fragmenata m/z, a kod n-alkana i molekulskog iona.

Abstract (english)

Revalidation of a method used for analyzing ignitable liquids was preformed. Method involves using SPME fiber for sampling and GC/MS coupled system for analysis. In the process of revalidation parameters that were reviewed are: SPLIT and SPLITLESS mode of gas chromatograph, sample heating time, sampling time, GC-column heating rate, limit of detection for gasoline and diesel fuel (LOD) and matrix influence. Organic compounds present in gasoline and diesel were identified. During the identification following criteria were valued: S/N≥3, QM≥70,presence of characteristic ion fragments m/z, and in the case of n-alkanes also the presence of molecule ion.

• Keywords: SPME-GC/MS metoda; revalidacija; lakozapaljive tekućine; alkani; aromatski ugljikovodici
• Keywords (english): SPME-GC/MS method; revalidation; ignitable liquids; alkanes; aromatic hydrocarbons
• Language: croatian
• URN:NBN: urn:nbn:hr:159:543552
• Study programme: Title: Biotechnology; specializations in: Biochemical-Microbiology; Course: Biochemical-Microbiology; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki (diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-09-01 07:23:16