Genotoksični učinak triazola na bakterijskom test sustavu i na kulturi stanica

master's thesis

• Title (english): Genotoxic effect of triazole on bacterial test system and human cell lines
• Author: Ivana Vrbanić
• Mentor: Ksenija Durgo (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2009-12-16, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Svaki novosintetizirani spoj prije nego što uđe u upotrebu mora proći genotoksikološka istraživanja kako bi se utvrdilo da je neškodljiv za ljude i okoliš. Istraživanjem triazola na bakterijskom test sustavu Salmonella typhimurium uočeno je da najviše istraživane koncentracije spoja izazivaju mutacije okvira čitanja te djeluju citotoksično sa ili bez dodatka metaboličkog aktivatora. Istraživan je utjecaj spoja na humanim stanicama raka grkljana, HEp2 i metastatskim stanicama debelog crijeva, SW. Neutral red testom i metodom određivanja aktivnosti mitohondrijske dehidrogenaze uočeno je da visoka koncentracija spoja izaziva destabilizaciju membrane u obje stanične linije te da smanjuje aktivnost mitohondrijske dehidrogenaze. Uočeno je da spoj inducira glutation. Spoj podliježe biotransformacijskim reakcijama, no pritom ne nastaju slobodni radikali, stoga nije uočena lipidna peroksidacija. Spoj u najvišim koncentracijama ima učinak na eukariotske stanice, a osim DNA molekule, mete djelovanja su proteini i membrane.

Abstract (english)

Every newly synthesized compound, before entering in common use, has to be tested for genotoxic properties so we can be sure that it is safe for human beings and enviroment. Investigated triazole caused frameshift mutation on bacterial test system Salmonella typhimurium and it was noted that the higest concentration of compound is cytotoxic, with or without metabolic activator. Influence of triazole on human larynx carcinoma cell line, HEp2 and metastatic colon cell line, SW was also examined. Neutral red assay and MTT test showed that the higest concentration of triazole caused destabilization of cell membrane in both cell lines and activity of mitohondrial deyidrogenase is decreased. Triazole induced glutathione pointing out that it goes through biotransformation reactions. Triazole did not induce free radical production or lipid peroxidation. At the higest concentration, triazole has an influence on DNA, proteins and membranes.

• Keywords: triazol; Ames-ov test; stanične linije; lipidna peroksidacija; glutation
• Keywords (english): triazole; Ames assay; cell lines; lipid peroxidation; glutathione
• Language: croatian
• URN:NBN: urn:nbn:hr:159:217604
• Study programme: Title: Biotechnology; specializations in: Biochemical-Microbiology; Course: Biochemical-Microbiology; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki (diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-09-01 09:23:27