Utjecaj koncentracije inokuluma i brzine miješanja na rast i produkciju rekombinantnog soja CHO stanica na kemijski definiranom mediju

master's thesis

• Title (english): Effect of inoculum concentration and stirring on growing and production capabilities of recombinant CHO cells in chemically defined media
• Author: Jasminka Bajić
• Mentor: Zlatko Kniewald (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2008-07-17, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U ovom radu proučavan je utjecaj koncentracije inokuluma i brzine miješanja na rast rekombinantnih CHO stanica i produkciju eritropoietina. Rekombinantne CHO stanice su adaptirane na rast u suspenziji i kemijski definiranom mediju. Provodio se šaržni uzgoj u spiner bocama. Eksperimenti su planirani, provedeni i analizirani EVOP metodom. Značajnost dvaju ulaznih faktora ocijenjena je na ovih osam izlaznih veličina: specifičnu brzinu rasta, specifičnu brzinu odumiranja, specifičnu brzinu potrošnje glukoze, specifičnu brzinu stvaranja laktata, specifičnu brzinu stvaranja produkta eritropoietina, koncentraciju eritropoietina, specifični prinos i volumetrijsku produktivnost.

Abstract (english)

During this work it has been studied effect of inoculum and stirring on growth of recombinant CHO cells and production of erythropoietin. Recombinant CHO cells have been adapted to growth in suspension and chemically defined media. Batch culture has been performed in spinner. Experiments have been designed, performed and analyzed by the EVOP method. Significant of this two input factors has been valued by eight output measures: specific growth rate, specific death rate, specific glucose spending rate, specific lactate producing rate, specific rate of producing erythropoietin product, concentration of erythropoietin, specific yield and volumetric productivity.

• Keywords: CHO stanice; eritropoietin; optimiranje uzgoja; planiranje pokusa; EVOP metoda
• Keywords (english): CHO cells; erythropoietin; optimization of cultivation; design of experiment; EVOP method
• Language: croatian
• URN:NBN: urn:nbn:hr:159:039020
• Study programme: Title: Biotechnology; specializations in: Biochemical-Microbiology; Course: Biochemical-Microbiology; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki (diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-09-01 13:05:03