Duplikacije ciljnog kromosoma prilikom transformacije kvasca Saccharomyces cerevisiae pomoću litijevog acetata

master's thesis

• Title (english): Targeted chromosome duplication during lithium acetate transformation of yeast Saccharomyces cerevisiae
• Author: Marina Miklenić
• Mentor: Zoran Zgaga (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2008-07-11, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U kvascu Saccharomyces cerevisiae, za razliku od ostalih eukariotskih organizama, nereplikativna transformirajuća DNA integrira se u genom mnogo češće homolognom, nego ilegitimnom rekombinacijom. To čini kvasac prikladnim modelnim organizmom za istraživanje rekombinacije ˝ends-out˝ (zamjene gena). Međutim, čak niti u kvascu, zamjena gena nije uvijek uspješna, već se pojavljuje i određen broj aberantnih genetičkih događaja, uključujući duplikacije ciljnog kromosoma. No, međutim, moguće je da je dio transformanata sa ovim genetičkim događajem nastao fuzijom stanica kvasca uslijed metode transformacije protoplastiranjem. Upotrebom alternativne metode transformacije, pomoću litijevog acetata, u ovom je radu pokazano da se duplikacija kromosoma prilikom zamjene gena događa bez obzira na metodu transformacije.

Abstract (english)

Unlike other eukaryotic organisms, in yeast Saccharomyces cerevisiae a non-replicative transforming DNA is much more often integrated into genome by homologous than by illegitimate recombination. That makes yeast an appropriate model organism for ends-out recombination (gene replacement) research. However, even in yeast, gene replacement isn't always successful and a number of aberrant genetic events can arise, including targeted chromosome duplication. However, it is posible that a portion of observed transformants with targeted chromosome duplication is in fact caused by fusion of yeast cells prompted by transformation of spheroplasts. Using an alternative transformation method, the lithium acetate method, this work shows that chromosome duplication occurs during gene replacement regardless of transformation method.

• Keywords: zamjena gena; rekombinacija ˝ends out˝; duplikacija kromosoma; kvasac
• Keywords (english): gene replacement; recombination ˝ends-out˝; chromosome duplication; yeast
• Language: croatian
• URN:NBN: urn:nbn:hr:159:944048
• Study programme: Title: Biotechnology; specializations in: Biochemical-Microbiology; Course: Biochemical-Microbiology; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki (diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-09-04 07:52:05