Istraživanje genotoksičnog učinka ekstrakta zelenog čaja na stanice humanog karcinoma grkljana

master's thesis

• Title (english): Investigation of genotoxic effect of green tea extract on human larynx carcinoma cells
• Author: Sandra Kostić
• Mentor: Ksenija Durgo (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2008-05-07, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Zeleni čaj, koji je vrlo popularno piće, proizvodi se iz biljke Camellia sinensis i bogat je flavonoidima za koje se smatra da imaju značajnu ulogu u sprečavanju nastanka raka. Kao najvažniji mehanizmi djelovanja flavonoida najčešće se spominju vezanje slobodnih radikala te suprimiranje formiranja reaktivnih kisikovih skupina. Cilj ovog rada bio je istražiti potencijalni genotoksični učinak ekstrakta zelenog čaja te epigalokatehin galata (EGCG) i epikatehin galata (ECG), flavonoida koji se u čaju nalaze u najvećoj koncentraciji. Dobiveni rezultati su pokazali da EGCG i ekstrakt zelenog čaja izazivaju povećanu citotoksičnost otporne stanične linije CK2 nakon kraće inkubacije. Produljenjem vremena inkubacije povećava se citotoksičnost istraživanjih spojeva. Također, ekstrakt zelenog čaja izaziva lipidnu peroksidaciju u CK2 stanicama. Istraživani katehini smanjuju bazalno primarno oštećenje DNA u roditeljskim HEp2 stanicama, dok ekstrakt zelenog čaja tijekom produljene inkubacije izaziva jednolančane i dvolančane lomove. Istraživani spojevi ne izazivaju oštećenja u CK2, rezistentnoj staničnoj liniji.

Abstract (english)

Green tea, widely consumed beverage, is produced from the plant Camellia sinensis and contains flavonoids that are considered to play an important role in cancer prevention. The most important mechanisms of their action are scavenging free radicals and suppressing ROS (reactive oxygen species) formation. The aim of this study was to evaluate potential genotoxic effect of green tea extract, epigallocatechin gallate (EGCG) and epicatechin gallate (ECG), flavonoids that are most abundant in tea. The results of this study demonstrate that EGCG and green tea extract induce increased genotoxicity of resistant CK2 cell line after shorter incubation time. Investigated compounds show higher degree of citotoxicity in longer period of incubation. Green tea extract induces lipid peroxidation in CK2 cell line. Investigated catechins reduce basal primary DNA damage in HEp2 cells, whereas green tea extract, after prolonged incubation, induces single and double strand breaks. Investigated compounds don't induce any damage in CK2 cell line.

• Keywords: citotoksičnost; ECG; EGCG; komet test; zeleni čaj
• Keywords (english): citotoxicity; comet assay; ECG; EGCG; green tea
• Language: croatian
• URN:NBN: urn:nbn:hr:159:414993
• Study programme: Title: Biotechnology; specializations in: Biochemical-Microbiology; Course: Biochemical-Microbiology; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki (diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-09-04 11:37:53