Utjecaj izvora ugljika na proizvodnju mliječne kiseline s pomoću bakterija Lactobacillus delbrueckii i Lactobacillus amylovorus DSM 20531T

master's thesis

• Title (english): Effect of carbon source on production of lactic acid by bacteria Lactobacillus delbrueckii and Lactobacillus amylovorus DSM 20531T
• Author: Ana Batušić
• Mentor: Anita Slavica (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2008-05, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U ovom je radu proveden uzgoj bakterije Lactobacillus delbrueckii u kemijski definiranim podlogama s dva izvora ugljika (glukoza i saharoza). Isto tako, istraživan je rast združene kulture bakterija L. delbrueckii i Lactobacillus amylovorus DSM 20531T u kemijski definiranoj podlozi sa škrobom. Uzgoj bakterijskih kultura proveden je u laboratorijskom bioreaktoru s miješalom šaržnim postupkom. Istraživanja su provedena kako bi se utvrdila učinkovitost fermentacije triju supstrata. Učinkovitost bioprocesa u kojima se proizvodi mliječna kiselina procijenjena je na osnovi analize tekućinskom kromatografijom visoke djelotvornosti (HPLC). L. delbrueckii fermentira glukozu i saharozu uz produktivnost ova dva bioprocesa od 1.82 g L-1 h-1 (glukoza) i 0.82 g L-1 h-1 (saharoza). Kod uzgoja združene kulture dviju bakterija u podlozi sa škrobom, bakterija L. amylovorus DSM 20531T hidrolizira škrob i nastali saharidi se fermentiraju s pomoću bakterija L. delbrueckii i L. amylovorus DSM 20531T.

Abstract (english)

In this investigation a cultivation of bacterium Lactobacillus delbrueckii was conducted in chemicaly defined media with two different carbon sources (glucose and sucrose). Likewise, the growth of a mixed culture, consisting of bacteria L. delbrueckii and Lactobacillus amylovorus DSM 20531T, has been investigated in chemicaly defined medium with starch as the carbon source. The batch cultivations were carried out in a lab-scale stirred tank bioreactor. The investigation was performed in order to define efficiency of fermentation of the three substrates. The estimation of the efficiency of the bioprocess in which lactic acid has been produced was based on high-pressure liquid chromatography (HPLC) analysis. L. delbrueckii degrades glucose and sucrose with the efficiency of 1.82 g L-1 h-1 and 0.82 g L-1 h-1, respectively. During the cultivation of the mixed culture in the medium with starch, bacterium L. amylovorus DSM 20531T hidrolizes starch and resulting saccharides were fermented by bacteria L. delbrueckii i L. amylovorus DSM 20531T.

• Keywords: mliječna kiselina; šaržni uzgoj; Lactobacillus delbrueckii; Lactobacillus amylovorus; HPLC
• Keywords (english): lactic acid; batch cultivation; Lactobacillus delbrueckii; Lactobacillus amylovorus; HPLC
• Language: croatian
• URN:NBN: urn:nbn:hr:159:263685
• Study programme: Title: Biotechnology; specializations in: Biochemical Engineering; Course: Biochemical Engineering; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo (diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-09-04 11:46:43