Nekonvencionalni pristupi za izravno određivanje likopena u proizvodima od rajčice

Lemić, Sonja

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master's thesis

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Few non-conventional approaches for direct assessment of lycopene in tomato-based foods: preliminary study

Sonja Lemić (2008)

University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Process Engineering
Laboratory for Measurement, Regulation and Automatisation

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APA 6th Edition

Lemić, S. (2008). Nekonvencionalni pristupi za izravno određivanje likopena u proizvodima od rajčice (Master's thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:322086

MLA 8th Edition

Lemić, Sonja. "Nekonvencionalni pristupi za izravno određivanje likopena u proizvodima od rajčice." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2008. https://urn.nsk.hr/urn:nbn:hr:159:322086

Chicago 17th Edition

Harvard

Lemić, S. (2008). 'Nekonvencionalni pristupi za izravno određivanje likopena u proizvodima od rajčice', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 21 August 2019, https://urn.nsk.hr/urn:nbn:hr:159:322086

Vancouver

Lemić S. Nekonvencionalni pristupi za izravno određivanje likopena u proizvodima od rajčice [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2008 [cited 2019 August 21] Available at: https://urn.nsk.hr/urn:nbn:hr:159:322086

IEEE

S. Lemić, "Nekonvencionalni pristupi za izravno određivanje likopena u proizvodima od rajčice", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2008. Available at: https://urn.nsk.hr/urn:nbn:hr:159:322086

Cite this item: https://urn.nsk.hr/urn:nbn:hr:159:322086

Metadata

Title	Nekonvencionalni pristupi za izravno određivanje likopena u proizvodima od rajčice
Title (english)	Few non-conventional approaches for direct assessment of lycopene in tomato-based foods: preliminary study
Author	Sonja Lemić
Mentor	Dane Bicanic (mentor)
Granter	University of Zagreb Faculty of Food Technology and Biotechnology (Department of Process Engineering) (Laboratory for Measurement, Regulation and Automatisation) Zagreb
Defense date and country	2008-02-18, Croatia
Scientific / art field, discipline and subdiscipline	BIOTECHNICAL SCIENCES Biotechnology
Abstract	Likopen , pigment je koji rajčici daje crvenu boju. Uobičajeno određivanje koncentracije likopena ostvaruje se klasičnim metodama poput HPLC-a i spektrofotometrije. Iako su metode apsolutne, zahtjevaju skupu i dugotrajnu ekstrakciju pigmenta prije same analize. U ovome radu, predložen je nov pristup određivanju koncentracije likopena. To su Fotoakusticna spektroskopija(FA) sa argon laserom, Fotoakustična spektroskopija(FA) sa Xe lampom i Rezonantna Ramanova spektroskopija. Osnovna ideja jest istraživanje moguće korelacije između koncentracije likopena i veličine signala mjerenih sa gore navedenim metodama. Različiti proizvodi bazirani na rajčicama su korišteni kao eksperimentalni uzorci. Rezultati FA i Raman mjerenja stavljeni su u odnos sa kolorimetrijskim indeksima L, a, b. Odlična korelacija postignuta je između FA signala (Xe lampom ili laserom) i koncentracije likopena izmjerenom putem HPLC-a. Isto vrijedi za rezultate postignute Ramanovom tehnikom. Nažalost korelacija izmedu L, a, b indeksa i koncentracije likopena nije tako dobra. U ovom trenutku razlozi za opaženu diskrepanciju nisu poznati.
Abstract (english)	Lycopene, the red pigment naturally present in tomatoes. Concentration of lycopene is usually determined by the absolute methods such as HPLC and spectrophotometry, but the extraction procedure of lycopene is a time consuming, destructive and costly. In this study a new approach to measurement of lycopene is presented. The newly proposed methods used here are the Photoacoustic spectroscopy(PA) with argon laser, Photoacoustic spectroscopy (PA) with Xe lamp and Resonance Raman spectroscopy. Various tomato-based products were used as test samples; all of them contain lycopene as a dominant colorant. This research study shows a good correlation between the spectroscopic techniques and lycopene content. Unfortunately the correlation between the colorimetric indices (a, b and L*) and the concentration of lycopene was rather poor.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:159:322086
Study programme	Title: Biotehnologija; smjerovi: Biokemijsko inženjerstvo Study programme type: sveučilišni Study level: dodiplomski Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo (diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo)
Type of resource	Text
File origin	Born digital
Access conditions	Restricted access
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Created on	2017-09-05 09:22:21