Određivanje melatonina u plodovima borovnice (Vaccinium corymbosum L.)

master's thesis

• Title (english): Determination of melatonin in blueberries (Vaccinium corymbosum L.)
• Author: Vesna Lukić
• Mentor: Verica Dragović-Uzelac (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Technology of Fruits and Vegetables Preservation and Processing); Zagreb
• Defense date and country: 2007-12-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

U ovom istraživanju analizirani su plodovi četiriju sorata borovnice (Vaccinium corymbosum L.), koji su brani u tri termina berbe tijekom dvije godine (2006, 2007). Cilj ovog istraživanja je utvrditi prisutnost i količinu biološkog spoja melatonina u plodovima borovnice. Određivanje melatonina provedeno pomoću dvije metode (metodom 1 i metodom 2). Metodom 1 provedena je ekstrakcija u uzorcima borovnice pomoću otapala metanola, a nakon ekstrakcije provedeno je pročišćavanje uzorka SPE ekstrakcijom, uz upotrebu C-18 kolonica. Metodom 2 ekstrakcija je provedena pomoću kalij-fosfatnog pufera (pH 8). Postupak je proveden bez pročišćavanja uzorka. Dobiveni ekstrakti iz obje metode analizirani su primjenom HPLC-FC. Nakon provrdene HPLC analize utvrđeno je da se u ekstraktima dobivenih metodom 1 gubi znatna količina melatonina, dok je u ekstraktima iz metode 2 melatonin određen u svim analiziranim sortama, u sva tri perioda berbe. Na temelju rezultata analize varijance utvrđeno je da ne postoje statistički značajne razlike u količini melatonina obzirom na istraživane sorte (Duke, Elliot, Sierra, Bluecrop), te obzirom na godinu berbe (2006,2007) (p<05).

Abstract (english)

In this research four different blueberry cultivars (Vaccinium corymbosum L ) collected during two years of harvesting (2006, 2007), were analyzed. The aim of this research was to determine the amount of biologically active compound melatonin in blueberry fruits. Melatonin was determined by using two methods (Method 1 and Method 2). According to Method 1 extraction was made by using methanol, and after extraction cleaning of extract using SPE extraction on C-18 column was performed. According to Method 2 extraction was made by using potassium phosphate puffer (pH 8) without extract cleaning. Obtained extracts were analyzed using HPLC-FD. After HPLC analysis it was observed that in extraction made by Method 1 losses of melatonin were high. Method 2 was better for isolation of melatonin. Method 2 was better for isolation of melatonin-melatonin was thus determined in all cultivare dealt with in the research and throughout harvesting period. Following the results of statistical variance analysis it has been determined that there is no significant statistical difference in the amount of melatonin within the different cultivar (Duke, Elliot, Sierra, Bluecrop) and having considered different years of harvesting (2006, 2007) (p<05).

• Keywords: borovnica; HPLC-FD; melatonin; Vaccinium corymbosum L.
• Keywords (english): blueberry; HPLC-FD; melatonin; Vaccinium corymbosum L.
• Language: croatian
• URN:NBN: urn:nbn:hr:159:027348
• Study programme: Title: Food Technology; specializations in: Food Engineering; Course: Food Engineering; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer prehrambene tehnologije; smjer prehrambeno inženjerstvo (diplomirani inženjer prehrambene tehnologije; smjer prehrambeno inženjerstvo)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-09-05 11:37:47