Karakterizacija polimera za pegilaciju rekombinantnih proteina metodom tekućinske kromatografije visoke djelotvornosti

master's thesis

• Title (english): Polymers characterisation for pegylation of recombinated proteins with high performance liquid chromatography method
• Author: Marina Šteib
• Mentor: Jasna Vorkapić-Furač (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); Zagreb
• Defense date and country: 2007-12-21, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U ovom radu primjenom tekućinske kromatografije visoke djelotvornosti (HPLC) i Evaporative light scattering detektora (ELSD) dokazane su molarne mase standarda (PEO 70000, PEO 30000, PEG 10000, PEG 4000 i PEG 1000). Pomoću poznatih masa standarda i njihovih vremena zadržavanja, koje je određeno iz snimljenih kromatograma, konstruirana je kalibracijska krivulja iz koje je moguće odrediti molarnu masu nepoznatog uzorka (npr. metoksipolietilen-glikol). Primjenom navedene metode vrši se karakterizacija polimera za pegilaciju rekombinantnih proteina koji se rabe u biotehnologiji i u farmaceutske svrhe.

Abstract (english)

In this thesis by using High Performance Liquid Chromatography and Evaporative Light Scattering Detector molar masses of following standards: PEO 70000, PEO 30000, PEG 10000, PEG 4000 i PEG 1000 were estimated. According to known molar masses and retention times, being defined from scanned chromatogram, a calibration curve was made. From calibration curve molar mass of an unknown sample (eg. methoxypolyethylene glycol) can be defined. By implementation of this method some characteristics of polymers for pegylation of recombinated proteins, being used in Biotechnology and Pharmacy, could be determined.

• Keywords: ELSD detektor; pegilacija; polietilen-glikol; tekućinska kromatografija visoke djelotvornosti
• Keywords (english): Evaporative Light Scattering Detector; High Performance Liquid Chromatography; pegylation; polyethylene glycol
• Language: croatian
• URN:NBN: urn:nbn:hr:159:331614
• Study programme: Title: Biotechnology; specializations in: Biochemical-Microbiology; Course: Biochemical-Microbiology; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki (diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-09-06 07:22:05