Bisorpcija iona cinka u bakterija mliječne kiseline

master's thesis

• Title (english): Bisorption of Zn 2+ in lactic acid bacteria
• Author: Jerko Penić
• Mentor: Vesna Stehlik Tomas (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Fermentation and Yeast Technology); Zagreb
• Defense date and country: 2007-10-03, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U ovom radu je ispitivana mogućnost vezanja iona cinka procesima biosorpcije u bakterije mliječne kiseline: Lactobacillus plantarum L73, Lactobacillus brevis L62 i Leuconostoc mesenteroides L3. Procesi biosorpcije iona cinka praćeni su pri statičkim i dinamičkim uvjetima uzgoja uz dodatak početne koncentracije cinka u rasponu od 0 – 80 mg/L te 0,8 g/L s. tv. bakterijske biomase. Rezultati su pokazali da je bakterija Leuconostoc mesenteroides L3 najefikasnija u procesu vezanja iona cinka te da miješanje tijekom procesa biosorpcije ne utječe na količinu vezanih iona cinka. S druge strane, pokazalo se da temperatura ima pozitivan učinak na vezanje iona cinka u biomasu bakterije Leuconostoc mesenteroides L3. Povećanjem koncentracije biomase, linearno se povećavala masa vezanog cinka, dok je vezanje iona cinka u bakterijsku biomasu tokom inkubacije pokazalo logaritamsku ovisnost. Prisutnost glukoze prilikom procesa biosorpcije iona cinka inhibirala je vezanje iona cinka u bakterijsku biomasu. U procesu vezanja iona cinka u BMK sudjeluju slobodne hidroksilne skupine polisaharida te proteini stanične stijenke.

Abstract (english)

In this work, possibility of binding Zn2+ ions, by processes of biosorption, in lactic acid bacteria Lactobacillus plantarum L73, Lactobacillus brevis L62 and Leuconostoc mesenteroides L3 was researched. Processes of biosorption of Zn2+ ions were performed in static and dinamic conditions. The achived results have shown that bacteria Leuconostoc mesenteroides L3 was the most effective in process of binding zinc ions and there was no significant difference between binding Zn2+ ions in static and dinamic conditions. On the other hand, it was shown that higher temperature is more suitable for accumulation of zinc ions into lactic acid bacteria. The biosorption of Zn2+ ions into bacterial biomass showed linear pattern. When following Zn2+ ions binding into lactic acid bacteria over time, the logarithmic pattern was obtained. By adding glucose during process of biosorption of Zn2+ ions, the uptake of zinc ions was inhibited. Free hydroxyl groups of polysaccharydes and proteins of the cell wall are involved in process of binding Zn2+ ions into LAB.

• Keywords: bakterije mliječne kiseline; cink; mikroelementi
• Keywords (english): lactic acid bacteria; microelements; zinc
• Language: croatian
• URN:NBN: urn:nbn:hr:159:774273
• Study programme: Title: Biotechnology; specializations in: Biochemical-Microbiology; Course: Biochemical-Microbiology; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki (diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-09-06 08:07:40