Uloga površinskih proteina tijekom preživljavanja probiotičkih sojeva u simuliranim uvjetima gastrointestinalnog trakta

undergraduate thesis

• Title (english): The role of surface proteins during survival of probiotic strains in simulated conditions of gastrointestinal tract
• Author: Ivona Jakša
• Mentor: Jagoda Šušković (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology); Zagreb
• Defense date and country: 2015-09-10, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology; Biology

Abstract

Cilj ovoga rada bio je ispitati ulogu površinskih S-proteina u in vitro preživljavanju Lactobacillus sojeva tijekom inkubacije u simuliranim uvjetima gastrointestinalnog trakta (GIT), te istražiti njihovu ulogu u in vitro adheziji bakterijskih stanica na proteine ekstracelularnog matriksa. Prema dobivenim rezultatima, dokazana je zaštitna uloga S-proteina u preživljavanju Lactobacillus sojeva u simuliranim uvjetima GIT-a. Kod svih ispitanih sojeva sposobnost preživljavanja u navedenim uvjetima je vidljivo smanjena nakon uklanjanja S-proteina s površine bakterijskih stanica, gvanidin hidrokloridom. Dokazana je također uloga S-proteina u povećanoj sposobnosti adhezije na proteine ekstracelularnog matriksa. Uklanjanje S-proteina uzrokovalo je smanjenje adhezije svih sojeva na kolagen, laminin i fibronektin. Sposobnost adhezija stanica soja L. plantarum D13 koji ne eksprimira S-proteine znatno je manja u usporedbi sa sojevima koji sadrže S-proteine na površini stanica.

Abstract (english)

The aim of this study was to examine the role of surface S-proteins in in vitro survival of Lactobacillus strains during incubation in simulated conditions of gastrointestinal tract, and to examine their role in in vitro adhesion of bacterial cells to extracellular matrix proteins. The results have proved the protective role of S-proteins in survival of Lactobacillus strains in simulated conditions of GIT. All of examined strains had lower survival abilities in mentioned conditions after removal of S-proteins from the surface of bacterial cells, using guanidine hydrochloride. The role of S-proteins in better adhesion ability to extracellular matrix proteins, have also been proved. Removal of S-proteins caused a reduction in the adhesion of all strains to collagen, laminin and fibronectin. The binding capability of the Lactobcillus plantarum D13 strain, which doesn't contain S-proteins, is much lower compared to strains that contain S-proteins on cell surface.

• Keywords: S-proteini; gastrointestinalni trakt; adhezija
• Keywords (english): S-proteins; gastrointestinal tract; adhesion
• Language: croatian
• URN:NBN: urn:nbn:hr:159:914077
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2015-12-15 11:14:44