Priprava prirodnih eutektičnih otapala i njihova primjena u krioprotekciji stanične linije

master's thesis

• Title (english): Preparation of natural eutectic solvents and their application in cryoprotection of cell lines
• Author: Natalia Ivanjko
• Mentor: Igor Slivac (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2016-09-28, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Prirodna eutektična otapala (eng. Natural Deep Eutectic Solvents, NADES) su nova generacija ekološki prihvatljivih otapala sa mogućim primjenama u različitim industrijskim područjima. Definiraju se kao smjesa dviju ili više komponenata koje se međusobno mogu povezati vodikovim vezama te koje u određenom omjeru imaju niže talište nego pojedinačne komponente smjese. S obzirom na njihovu široku primjenu, u ovom radu ispitana je mogućnost njihove primjene kao krioprotektanata na staničnoj liniji HEK 293T. U ovom radu pripravljena su dva različita NADES-a u različitim molarnim odnosima (kolin klorid:glukoza, prolin:glukoza) te s različitim udjelima vode. Učinkovitost ovih NADES-a kao krioprotektanata uspoređena je sa standardnim krioprotektantima, glicerolom i dimetil sulfoksidom. Istraživanje je pokazalo da testirani spojevi nisu dostojna zamjena standardnim kriprotektantima.

Abstract (english)

Natural deep eutectic solvents (NADES) are new generation of environmentally friendly solvents with potential application in various industrial fields. They are defined as a mixture of two or more components connected to each other by hydrogen bonds. At a certain ratio of their building components, NADES can have lower melting points than the individual components of the mixture. Considering their widespread application, NADES are tested as cryoprotectants on cell line HEK 293T. Two different NADES are made for this experiment; they are consisted of proline/glucose and choline chloride/glucose in different molar ratio and with different percentage of water content. Efficiency of those NADES are compared with standard cryoprotectants, glycerol and dimethyl sulfoxide. The results showed that NADES cannot serve as an appropriate alternative to the standard cryoprotectants.

• Keywords: prirodna eutektična otapala; fizikalno–kemijska svojstva; krioprotektanti; citotoksičnost
• Keywords (english): natural deep eutectic solvents; physico-chemical properties; cryoproctentants; cytotoxity
• Language: croatian
• URN:NBN: urn:nbn:hr:159:232952
• Study programme: Title: Food Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka prehrambenog inženjerstva (magistar/magistra inženjer/inženjerka prehrambenog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-25 10:19:25