Procjena učinka pušenja na genom ljudskih limfocita izloženih γ-zračenju primjenom testa mikronukleusa i testa lomljivosti kromatida

master's thesis

• Title (english): Estimation of smoking effect on genome of human lymphocytes exposed to γ-radiation using micronucleus assay and chromatid breakage assay
• Author: Jovica Lončar
• Mentor: Verica Garaj-Vrhovac (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2005-05-06, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Primjenom testa mikronukleusa i testa lomljivosti kromatida istraženi su genotoksički učinci pušenja na genom ljudskih limfocita u spontanim in vivo uvjetima i γ-zračenjem induciranim in vitro uvjetima. Nadalje je učinjena procjena utjecaja spola na oštećenost genoma te procjena razlika u radioosjetljivosti. Testom mikronukleusa utvrđen je statistički značajno veći stupanj oštećenja genoma pušača od stupnja oštećenja genoma nepušača. Utjecaj spola nije pronađen. Testom lomljivosti kromatida utvrđeno je da se broj lomova po stanici, kao i broj stanica s lomom, između pušača i nepušača statistički značajno ne razlikuju. Velike individualne razlike u radioosjetljivosti dobivene testom mikronukleusa nisu potvrđene testom lomljivosti kromatida. Stoga se ne može izvesti jednoznačan zaključak.

Abstract (english)

Using micronucleus assay and chromatid breakage assay we investigated genotoxicity of smoking on genome of human lymphocytes in spontaneous in vitro conditions and γ-radiation induced in vivo conditions. Furthermore we estimated the sex effect in damaging the genome and estimated the differences in radiosensitivity. Micronucleus assay established that the degree of damage of genome in smokers’ lymphocytes was higher compared to nonsmokers and the difference reached statistical significance. Sex effect was not found. Chromatid breakage assay didn’t find statistically significant difference in breaks per cell, as well as in number of cells with break, between smokers and nonsmokers. Big interindividual differences in radiosensitivity established with micronucleus assay were not confirmed by chromatid breakage assay. Therefore we can not make univocal conclusion.

• Keywords: genotoksičnost pušenja; γ-zračenje; radioosjetljivost; test mikronukleusa; test lomljivosti kromatida
• Keywords (english): chromatid breakage assay; genotoxicity of smoking; γ-radiation; micronucleus assay; radiosensitivity
• Language: croatian
• URN:NBN: urn:nbn:hr:159:444060
• Study programme: Title: Biotechnology; specializations in: Biochemical-Microbiology; Course: Biochemical-Microbiology; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki (diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-09-12 07:44:01