Title | Uloga translezijskih polimeraza u inaktivaciji profuga |
Author | Siniša Bratulić |
Mentor(s) | Višnja Bačun-Družina (thesis advisor)
|
Abstract | Provedena je genetička analiza inaktivacije profaga λ u bakterijama Escherichia coli ozračenim UV svjetlom. Cilj je bio ustanoviti povezanost aktivnosti translezijskih polimeraza PolB, UmuDC, DinB, te helikaze DinG sa procesom koji se događa u ozračenim bakterijama, a koji onesposobljava profag za mjesno-specifičnu rekombinaciju s bakterijskim kromosomom, odnosno za izrezivanje iz kromosoma. Pokazalo se da mutanti polB, dinB, umuCD, kod kojih nema aktivnosti translezijske sinteze, kao i mutant dinG, u postiradijacijskoj inkubaciji pokazuju pojavu progresivnog pada sposobnosti stvaranja plakova, odnosno pojavu inaktivacije profaga. Kinetika inaktivacije profaga je kod svih mutanata bila slična kao i kod stanica divljeg tipa. Ovi rezultati pokazuju da fenomen inaktivacije profaga u postiradijacijskoj inkubaciji ne ovisi o translezijskoj sintezi ni o aktivnosti inducibilne helikaze DinG, već je posljedica neuspješnog rekombinacijskog popravka. |
Keywords | DinG Escherichia coli prophage λ recombination translesion synthesis |
Parallel title (English) | Role of translesion polymerases in prophage inactivation |
Granter | University of Zagreb Faculty of Food Technology and Biotechnology |
Lower level organizational units | Department of Biochemical Engineering |
Place | Zagreb |
State | Croatia |
Scientific field, discipline, subdiscipline | BIOTECHNICAL SCIENCES Biotechnology
|
Study programme type | university |
Study level | graduate |
Study programme | Biotechnology; specializations in: Biochemical-Microbiology |
Study specialization | Biochemical-Microbiology |
Academic title abbreviation | dipl. ing. bioteh. |
Genre | master's thesis |
Language | Croatian |
Defense date | 2004-11-11 |
Parallel abstract (English) | Genetic analysis of λ prophage inactivation in UV-irradiated Escherichia coli was carried out. The aim of this work was to establish a connection translesion sythesis activities of PolB, UmuDC, DinB polymerases, and helicase DinG with the process that takes place in irradiated bacteria, and which makes the prophage incapable of site-specific recombination, i.e. excision from the host chromosome. It was shown that progressive loss of plaque forming ability during postirradiation incubation, i.e. prophage inactivation, occured in mutants carrying polB, dinB, umuCD, which do not exhibit translesion synthesis, as well as dinG mutant. In all mutants, kinetics of prophage inactivation was similar to the one shown by the wild type. These results show that the phenomenon of prophage inactivation during postiradiation incubaction is not dependent on translesion synthesis or inducible DinG helicase activity, but is a consequence of unsuccessful recombinational repair. |
Parallel keywords (Croatian) | DinG Escherichia coli profag λ rekombinacija translezijska sinteza |
Resource type | text |
Access condition | Access restricted to students and staff of home institution |
Terms of use |  |
URN:NBN | https://urn.nsk.hr/urn:nbn:hr:159:558558 |
Committer | Valter Ilić |