Uloga translezijskih polimeraza u inaktivaciji profuga

master's thesis

• Title (english): Role of translesion polymerases in prophage inactivation
• Author: Siniša Bratulić
• Mentor: Višnja Bačun-Družina (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); Zagreb
• Defense date and country: 2004-11-11, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Provedena je genetička analiza inaktivacije profaga λ u bakterijama Escherichia coli ozračenim UV svjetlom. Cilj je bio ustanoviti povezanost aktivnosti translezijskih polimeraza PolB, UmuDC, DinB, te helikaze DinG sa procesom koji se događa u ozračenim bakterijama, a koji onesposobljava profag za mjesno-specifičnu rekombinaciju s bakterijskim kromosomom, odnosno za izrezivanje iz kromosoma. Pokazalo se da mutanti polB, dinB, umuCD, kod kojih nema aktivnosti translezijske sinteze, kao i mutant dinG, u postiradijacijskoj inkubaciji pokazuju pojavu progresivnog pada sposobnosti stvaranja plakova, odnosno pojavu inaktivacije profaga. Kinetika inaktivacije profaga je kod svih mutanata bila slična kao i kod stanica divljeg tipa. Ovi rezultati pokazuju da fenomen inaktivacije profaga u postiradijacijskoj inkubaciji ne ovisi o translezijskoj sintezi ni o aktivnosti inducibilne helikaze DinG, već je posljedica neuspješnog rekombinacijskog popravka.

Abstract (english)

Genetic analysis of λ prophage inactivation in UV-irradiated Escherichia coli was carried out. The aim of this work was to establish a connection translesion sythesis activities of PolB, UmuDC, DinB polymerases, and helicase DinG with the process that takes place in irradiated bacteria, and which makes the prophage incapable of site-specific recombination, i.e. excision from the host chromosome. It was shown that progressive loss of plaque forming ability during postirradiation incubation, i.e. prophage inactivation, occured in mutants carrying polB, dinB, umuCD, which do not exhibit translesion synthesis, as well as dinG mutant. In all mutants, kinetics of prophage inactivation was similar to the one shown by the wild type. These results show that the phenomenon of prophage inactivation during postiradiation incubaction is not dependent on translesion synthesis or inducible DinG helicase activity, but is a consequence of unsuccessful recombinational repair.

• Keywords: DinG; Escherichia coli; profag λ; rekombinacija; translezijska sinteza
• Keywords (english): DinG; Escherichia coli; prophage λ; recombination; translesion synthesis
• Language: croatian
• URN:NBN: urn:nbn:hr:159:558558
• Study programme: Title: Biotechnology; specializations in: Biochemical-Microbiology; Course: Biochemical-Microbiology; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki (diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-09-12 10:06:24