Dinamika rasta stanica u CHO (Chinese Hamster Ovary)-K1 staničnoj liniji pod djelovanjem lindana

master's thesis

• Title (english): Cell growth dynamics in CHO (Chinese Hamster Ovary)-K1 cell line under lindane treatment
• Author: Lucija Šišina
• Mentor: Jasna Kniewald (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Toxicology); Zagreb
• Defense date and country: 2004-12-03, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Pesticidi osim korisnih učinaka u poljoprivrednoj proizvodnji imaju i mnoge štetne učinke koji se očituju u njihovu djelovanju na okoliš, te na zdravlje čovjeka. Za utvrđivanje toksičkih učinaka pesticida provode se klasični (in vivo) i alternativni (in vitro) testovi toksičnosti. U ovom radu je za određivanje citotoksičnosti insekticida lindana korištena stanična linija ovarija kineskog hrčka (CHO-K1). CHO-K1 stanice rastu u monosloju u Dulbecco¢s MEM mediju uz dodatak 10% FBS-a (fetal bovine serum), odnosno 10% NBS-a (newborn bovine serum). Citotoksičnost lindana na CHO-K1 stanice praćena je u rasponu koncentracija lindana od 10-100 mg/mL medija za uzgoj pri početnoj koncentraciji stanica od 2,5 x 104 st/mL medija. IC50 vrijednost u pokusu s FBS serumom određena metodom Neutral Red iznosi 36,24 µg lindana/mL medija za uzgoj, a IC50 vrijednost određena metodom Trypan Blue iznosi 16,17 µg lindana/mL medija za uzgoj. U pokusu s NBS serumom IC50 vrijednost određena metodom Trypan Blue iznosi 35,71 µg lindana/mL medija za uzgoj. Kolorimetrijske metode Neutral Red i Trypan Blue primjenjive su u ispitivanju citotoksičkog učinka ksenobiotika na CHO-K1 stanice.

Abstract (english)

Aside of their benefit effects in agricultural production, pesticides have many harmful effects, which exert their action on the environment, as well as on health of the human population. Standard (in vivo) and alternative (in vitro) tests are carried out to assess toxic effects of pesticides. CHO-K1, Chinese Hamster Ovary cell line was used during the course of this study to evaluate cytotoxicity of insecticide lindane. CHO-K1 cells grow in a monolayer in Dulbecco¢s MEM with addition of 10% of FBS (fetal bovine serum) or 10% of NBS (newborn bovine serum). Cytotoxic effect of lindane was evaluated by using a range of concentrations between 10 to 100 mg/mL of cultivating medium with the initial cell concentration of 2.5 x 104 cells/mL of cultivating medium. IC50 value determined in the FBS experiment using Neutral Red dye method was 36.24 µg lindane/mL of cultivating medium, while the IC50 value determined by using Trypan Blue dye method was 16.17 µg lindane/mL of cultivating medium. In the NBS experiment the IC50 value determined by using Trypan Blue dye method was 35.71 µg lindane/mL of cultivating medium. Both colorimetric methods Neutral Red and Trypan Blue are acceptable for the quantification of cytotoxicity of xenobiotics on CHO-K1 line.

• Keywords: lindan; CHO-K1 stanična linija; citotoksičnost; metode bojanja Neutral Red i Trypan Blue
• Keywords (english): lindane; CHO-K1 cell line; cytotoxicity; dyeing methods Neutral Red and Trypan Blue
• Language: croatian
• URN:NBN: urn:nbn:hr:159:490001
• Study programme: Title: Biotechnology; specializations in: Biochemical-Microbiology; Course: Biochemical-Microbiology; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki (diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-09-12 13:20:56