Konformacijske promjene proteina sirutke praćene FTIR spektroskopijom

master's thesis

• Title (english): Conformational changes of whey proteins monitored by FTIR spectroscopy
• Author: Slavica Moše
• Mentor: Damir Kovaček (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Analytical Chemistry); Zagreb
• Defense date and country: 2005-04-22, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U ovom radu praćene su konformacijske promjene proteina sirutke (koncentrat proteina sirutke, izolat proteina sirutke i β - laktoglobulin) pod utjecajem visokog tlaka od 300, 400, 500 i 600 MPa. Otkriveno je da povećanje tlaka uzrokuje denaturaciju sirutkinih proteina. Raspon promjena na proteinu uzrokovan visokim tlakom ovisi o strukturi nativnog proteina, primjenjenom tlaku i vanjskim faktorima kao što su temperatura, pH i ionska jakost. Ireverzibilne denaturacije koje dovode do agregacije i formiranja gela dolaze do izražaja pri tlakovima većim od 400 MPa.

Abstract (english)

The conformational changes of whey proteins (WPC, WPI, β - lactoglobuline) have been investigated at pressures of 300, 400, 500 and 600 MPa. It was discovered that high pressure causes denaturation of whey proteins. The range of changes on the protein caused by high pressure depends on structure of native protein, the pressure applied and outside factors, such as temperature and pH. Irreversible denaturation and gel formations appear at pressures higher than 400 MPa.

• Keywords: FT-IR; mlijeko; sirutka; visoki tlak
• Keywords (english): FT-IR; high pressure; milk; whey
• Language: croatian
• URN:NBN: urn:nbn:hr:159:423165
• Study programme: Title: Biotechnology; specializations in: Biochemical Engineering; Course: Biochemical Engineering; Study programme type: university; Study level: graduate; Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo (diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-09-13 10:54:28