Izolacija patogenih mikroorganizama riba i školjkaša porijeklom iz Jadranskog mora

undergraduate thesis

• Title (english): Isolation of pathogenic microorganisms found in fish and shellfish harvested from Adriatic sea
• Author: Petra Bosanac
• Mentor: Ksenija Markov (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for General Microbiology and Food Microbiology); Zagreb
• Defense date and country: 2015-07-08, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Mikrobi koji obitavaju na vodenim organizmima odražavaju status vode iz koje su uzeti te diktiraju konačnu kakvoću proizvoda. Kod riba mikrobi zauzimaju vanjski sluzavi omotač, škrge i probavni sustav, a kvarenje mesa školjkaša započinje na vanjskim površinama organizama. Mikroflora riba i školjkaša obuhvaća određenu mikrofloru autohtonih i patogenih mikroorganizama koji utječu na zdravlje ljudi. Stoga je cilj ovog rada bio izolirati patogene mikroorganizme iz lubina i školjkaša (dagnje i kamenice) porijeklom iz Jadranskog mora. Izolacija patogena je provedena iz različitih dijelova lubina koji su obuhvaćali sluz sa površine ribe, škrge, mišić i bubreg, sadržaj želuca, te crijeva i želudac, a kod dagnji i kamenica izolacija je provedena sa škrga, iz probavne žlijezde i želuca. Iz uzoraka su klasičnim mikrobiološkim metodama izolirane bakterije Listeria sp., sulfitoreducirajući klostridiji, Salmonella sp., Escherichia coli, Vibrio cholerae, Vibrio parahaemolyticus i Pseudomonas aeruginosa. Biokemijskim API testovima u uzorcima nije dokazana patogena bakterija Listeria monocytogenes već Listeria ivanovii.

Abstract (english)

Microbes that can be found in the large number of aquatic organisms reflect the status of the water and dictate the final product quality. In fish the microbes can occupay the external mucous layer, gills and digestive system, and they can also be found on the exterior of a shellfish organism. Microflora of fish and shellfish includes certain pathogenic bacteria that can affect human health. Therefore, the aim of this study was to isolate pathogenes from sea bass and shellfish (mussels and oysters) found in the Adriatic Sea. Isolation of the pathogen was carried out in different parts of seabass involving mucus from the surface of fish, gills, muscle and kidney, stomach contents( the stomach and intestines). In mussels and oysters isolation was performed with the gills of the digestive gland and stomach. Using classical microbiological methods we isolated Listeria sp., sulphite-reducing Clostridia, Salmonella spp., Escherichia coli, Vibrio cholerae, Vibrio parahaemolyticus and Pseudomonas aeruginosa. API biochemical test in samples was also performed,but we did not prove the pathogen Listeria monocytogenes,just the non pathogenic bacteria Listeria ivanovii.

• Keywords: lubin; kamenice; dagnje; patogeni mikroorganizmi; klasične mikrobiološke metode; API testovi
• Keywords (english): isolation; pathogenic microorganisms; sea bass; oysters; mussels; microbiological methods; API
• Language: croatian
• URN:NBN: urn:nbn:hr:159:954355
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-25 11:02:43