Preživljavanje prirodne mikrobiote izolirane iz proizvoda akvakulture u simuliranim uvjetima gastrointestinalog trakta

undergraduate thesis

• Title (english): Survival of natural microbias isolated from aquaculture products in simulated conditions of the gastrointestinal tract
• Author: Nikolina Matešić
• Mentor: Jadranka Frece (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for General Microbiology and Food Microbiology); Zagreb
• Defense date and country: 2017-09-19, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Mnogi bakterijski sojevi iz roda Lactobacillus i Bifidobacterium su okarakterizirani kao probiotici jer iskazuju pozitivne učinke na zdravlje domaćina. Preţivljavanje nepovoljnih uvjeta gastrointestinalnog trakta (GIT) je jedan od ključnih kriterija prilikom odabira probiotičkih sojeva budući da je GIT ciljno mjesto njihovog djelovanja. U ovome radu ispitano je preţivljavanje autohtonih izolata BMK iz riba i školjaka Jadranskog mora-Lactobacillus plantarum O1, Lactobacillus helveticus O9, Leuconostoc mesenteroides L4A, Lactobacillus plantarum D1 i Lactobacillus plantarum K4 u simuliranim uvjetima GIT-a. Svi bakterijski sojevi pokazuju visoku otpornost prema djelovanju pepsina te dobro podnose visoke koncentracije vodikovih iona, ali pokazuju i dobru toleranciju na djelovanje enzima gušterače i ţučnih soli. Bakterijski soj L. plantarum O1 pokazao je najbolje preţivljavanje u simuliranim uvjetima GIT-a, dok je najmanju sposobnost preţivljavanja imao bakterijski soj L. plantarum D1.

Abstract (english)

Many bacterial strains from Lactobacillus and Bifidobacterium genus are characterized as probiotics due to their positive effects on the health of the host. The survival of adverse conditions of the gastrointestinal tract (GIT) is one of the main criteria in the selection of probiotic strains because GIT is target location of their work. Autochthonous LAB strains isolated from fish and shellfish of Adriatic sea-Lactobacillus plantarum O1, Lactobacillus helveticus O9, Leuconostoc mesenteroides L4A, Lactobacillus plantarum D1 and Lactobacillus plantarum K4) were examined in this work for their ability to survive harsh simulated GIT conditions in vitro.All bacterial strains show high resistance to the activity of pepsin and to high concentration of hydrogen ions, but also they show good resistance to the activity of pancreatic enzymes and bile salts. Bacterial strain L. plantarum O1 showed highest survival in simulated GIT conditions, while the lowest survival was detected for the strain L. plantarum D1.

• Keywords: bakterije mliječne kiseline; probiotici; gastrointestinalni trakt
• Keywords (english): lactic acid bacteria; probiotics; gastrointestinal tract
• Language: croatian
• URN:NBN: urn:nbn:hr:159:375813
• Study programme: Title: Food Technology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-09-20 09:34:57