Determining survival of probiotic bacterial strains Bifidobacterium animalis subs. lactis Bb12 and Lactobacillus plantarum D13 immobilized in hydroxyethyl cellulose gel

undergraduate thesis

• Title: Određivanje preživljavanja probiotičkih sojeva bakterija Bifidobacterium animalis subs. lactis Bb12 i Lactobacillus plantarum D13 imobiliziranih u hidroksietil celuloznom gelu
• Author: Andrea Plec
• Mentor: Antonio Starčević (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Section for Bioinformatics); Zagreb
• Defense date and country: 2017-09-19, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Mikrobiota je mikrobna flora prisutna u i na ljudskom organizmu. Neravnoteža crijevne mikrobiote povezuje se s mnogim bolestima poput upalnih crijevnih bolesti, dijabetesa i raka, ali i pretilosti. Budući da su za zdrav mikrobiom zaslužni različiti mikroorganizmi kod različitih domaćina, za uravnoteženje crijevne mikrobiote koriste se raznovrsni probiotički pripravci. Probiotici su živi mikroorganizmi koji se primjenjuju kako bi donijeli zdravstvenu korist domaćinu, a za njihovo djelovanje postoji nekoliko predloženih mehanizama. Međutim, kako bi probiotici mogli djelovati potrebno je osigurati da živi dospiju u crijeva. U ovom radu ispitano je preživljavanje stanica dva probiotička soja, Bifidobacterium animalis subsp. lactis Bb12 i Lactobacillus plantarum D13, imobilizirana u hidroksietil celuloznom gelu. Nakon 30 dana držanja u gelu pokazalo se kako hidroksietil celulozni gel nije pogodno sredstvo za imobilizaciju i čuvanje ovih probiotičkih sojeva.

Abstract (english)

Microbiota is the microbial flora present in and on the human organism. Dysbiosis of the intestinal microbiota is associated with many diseases, such as inflammatory bowel disease, diabetes, cancer and obesity. Since a healthy microbiome consists of different microorganisms in different hosts, various probiotic formulas are used to restore the balance of the intestinal microbiota. Probiotics are live microorganisms which confer a health benefit on the host, with several proposed mechanisms of action. However, in order for probiotics to work, it’s necessary to ensure that they arrive in the gut alive. The aim of this work was to test the survival of two probiotic strains, Bifidobacterium animalis subsp. lactis Bb12 and Lactobacillus plantarum D13, which were immobilized in hydroxyethyl cellulose gel. After 30 days of preservation it was concluded that hydroxyethyl cellulose gel isn’t suitable for immobilization and preservation of mentioned probiotic strains.

• Keywords: Bifidobacterium animalis subsp. lactis Bb12; hidroksietil celulozni gel; Lactobacillus plantarum D13; mikrobiota; probiotici
• Keywords (english): Bifidobacterium animalis subsp. lactis Bb12; hydroxyethyl cellulose gel; Lactobacillus plantarum D13; microbiote; probiotics
• Language: croatian
• URN:NBN: urn:nbn:hr:159:293686
• Study programme: Title: Biotehnologija; Study programme type: sveučilišni; Study level: preddiplomski; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-09-25 07:45:59