Microbiota is the microbial flora present in and on the human organism. Dysbiosis of the intestinal microbiota is associated with many diseases, such as inflammatory bowel disease, diabetes, cancer and obesity. Since a healthy microbiome consists of different microorganisms in different hosts, various probiotic formulas are used to restore the balance of the intestinal microbiota. Probiotics are live microorganisms which confer a health benefit on the host, with several proposed mechanisms of action. However, in order for probiotics to work, it’s necessary to ensure that they arrive in the gut alive. The aim of this work was to test the survival of two probiotic strains, Bifidobacterium animalis subsp. lactis Bb12 and Lactobacillus plantarum D13, which were immobilized in hydroxyethyl cellulose gel. After 30 days of preservation it was concluded that hydroxyethyl cellulose gel isn’t suitable for immobilization and preservation of mentioned probiotic strains.