Određivanje udjela ukupnih fenolnih spojeva i antioksidacijske aktivnosti u bezglutenskom kiselom tijestu i kruhu s dodatkom brašna žutog graška

master's thesis

• Title (english): Determination of total phenolic content and antioxidant activity in gluten-free sourdough and bread with added yellow pea flour
• Author: Ivan Štoler
• Mentor: Marina Krpan (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Quality Control); (Laboratory for Food Quality Control); Zagreb
• Defense date and country: 2017-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Celijakija je autoimuni poremećaj od kojeg mogu oboljeti osobe s genetskom predispozicijom, a manifestira se oštećenjem tankog crijeva ukoliko oboljela osoba prehranom unese gluten. Bezglutenski kruh predstavlja određenu vrstu tehnološkog izazova s obzirom da gluten kao protein sudjeluje u formiranju strukture kruha te utječe na njegova senzorska svojstva. Uporabom mješavine brašna od sirovina koje ne sadrže gluten te tehnologije kiseljenja tijesta nastoje se poboljšati senzorska i nutritivna svojstva kruha. Cilj ovog rada bio je odrediti udio ukupnih slobodnih fenolnih spojeva TPC metodom i antioksidacijsku aktivnost DPPH i FRAP metodama na uzorcima bezglutenskog kiselog tijesta s dodatkom brašna žutog graška te kruha i kore kruha s dodatkom istog kiselog tijesta. Prema dobivenim rezultatima može se zaključiti da fermentacija tijesta s L. fermentum i L. brevis značajno utječe na povećanje udjela slobodnih fenolnih spojeva i antioksidacijske aktivnosti kiselog tijesta kao i kruha s njegovim dodatkom.

Abstract (english)

Celiac disease is an autoimmune disorder that can occur in genetically predisposed individuals where the ingestion of gluten leads to damage of the small intestine. Gluten-free bread production is a technological challenge given the fact that gluten has a role in forming the structure of bread, and has influence on its sensory characteristics. Flour or mix of flours from gluten-free seeds and plants and sourdough technology are used to improve sensory and nutritive properties of bread. The aim of this thesis was to determine the total free phenolic content (TPC) and antioxidative activity (DPPH and FRAP methods) of gluten-free sourdough made from yellow pea flour, as well as breadcrumb and crust made with the same sourdough. Results show that the fermentation of dough with addition of L. fermentum and L. brevis significantly increases the content of free phenolic compounds and antioxidative activity of sourdough bread and bread crust.

• Keywords: bezglutenski kruh; brašno žutog graška; DPPH; FRAP; TPC
• Keywords (english): DPPH; FRAP; gluten free; TPC; yellow pea flour
• Language: croatian
• URN:NBN: urn:nbn:hr:159:627133
• Study programme: Title: Food Safety Management; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane (magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-11-09 13:44:54