Određivanje alergena mlijeka ELISA metodom

master's thesis

• Title (english): Determination of milk allergens by ELISA method
• Author: Zrinka Tišlarić
• Mentor: Ksenija Marković (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Quality Control); (Laboratory for Food Quality Control); Zagreb
• Defense date and country: 2017-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Tijekom ovog istraživanja, imunoenzimskom ELISA (engl. Enzyme-Linked ImmunoSorbent Assay) metodom analiziran je udio potencijalnih alergena mlijeka u 24 uzorka keksa i čajnih peciva prikupljenih iz trgovačkih lanaca i slastičarnica sa zagrebačkog tržišta, te su prikupljeni stavovi i mišljenja potrošača o mlijeku i proizvodima od mlijeka kao tvarima ili proizvodima koji uzrokuju alergije. U 9 od 12 analiziranih uzoraka keksa i čajnih peciva iz trgovačkih lanaca detektirana je prisutnost alergena mlijeka pri čemu je prosječna vrijednost iznosila 0,58 mgkg-1, a uzorci s najvišim udjelom alergena mlijeka (0,70 mgkg-1) nisu u okviru informacija o hrani sadržavali navod vezan uz prisutnost alergena mlijeka. Udio alergena mlijeka, detektiranih u 3 od 5 analiziranih uzoraka keksa i čajnih peciva iz slastičarnica, iznosio je prosječno 0,14 mgkg-1 pri čemu informacije o hrani analiziranih proizvoda nisu pružale navode vezane uz prisutnost alergena mlijeka. U svih 7 analiziranih uzoraka keksa s maslacem iz trgovačkih lanaca i slastičarnica detektirani su alergeni mlijeka, pri čemu su niži udjeli određeni u uzorcima keksa s maslacem iz trgovačkih lanaca. 62,2 % ispitanika smatra kako keksi ili čajna peciva ne sadrže tragove mlijeka ukoliko na deklaraciji takovih proizvoda nema navoda o mogućoj prisutnosti mlijeka. Od 24 ukupno analizirana uzorka, ELISA metodom su alergeni mlijeka detektirani u 6 uzoraka bez navoda vezanih uz prisutnost alergena mlijeka u okviru informacija o hrani.

Abstract (english)

During this study, the content of potential milk allergens in 24 samples of biscuits and tea biscuits collected from supermarkts and pastry shops on Zagreb market was analysed by ELISA (Enzyme-Linked ImmunoSorbent Assay) method, and also the consumers opinion on milk and milk products as substances or products that caused allergies was examined. The presence of milk allergens was detected in 9 out of 12 analysed samples of biscuits and tea biscuits from supermarkets, with an average value of 0,58 mgkg-1, and food informations of samples with the highest value of milk alllergens (0,70 mgkg-1) were not included advisory label related to the presence of milk allergens. The average content of milk allergens, detected in 3 of the 5 analysed samples of biscuits and tea biscuits from pastry shops, was 0,14 mgkg-1, where the food information of the analysed products did not provide any indication of the presence of milk allergens. In all 7 analysed samples of butter biscuits from supermarkets and pastry shops, milk allergens were detected, with lower contents being determined in the samples from supermarkets. 62,2% of respondents consider that biscuits or tea biscuits do not contain traces of milk if declarations of products not provide informations of possible presence of milk. Among 24 analysed samples, by the ELISA method, milk allergens were detected in 6 samples without advisory label related to the presence of milk allergens within food information.

• Keywords: alergeni mlijeka; ELISA; informacija o hrani
• Keywords (english): milk allergens; ELISA; food information
• Language: croatian
• URN:NBN: urn:nbn:hr:159:710396
• Study programme: Title: Food Safety Management; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane (magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-11-13 11:19:44