Određivanje sadržaja bakterijskih endotoksina u injekcijama azitromicina

master's thesis

• Title (english): Determination of bacterial endotoxin content in azithromycin injection
• Author: Doris Delač
• Mentor: Ivana Radojčić Redovniković (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2017-07-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Sadržaj bakterijskih endotoksina u uzorcima injekcija azitromicina određen je kinetičkom turbidimetrijskom metodom i brzom metodom na prijenosnom spektrofotometru. Kinetička turbidimetrijska metoda je prije analize validirana ispitivanjem utjecaja uzorka na inhibiciju ili pojačanje vrijednosti u testu za određivanje bakterijskih endotoksina. Iako je kinetička turbidimetrijska metoda, metoda izbora za kvantitativno određivanje sadržaja bakterijskih endotoksina, brza metoda na prijenosnom spektrofotometru zahtjeva kraće vrijeme analize. Upravo zbog toga je potrebno usporediti ove dvije metode i dokazati da je i brza metoda na prijenosom spektrofotometru valjana.

Abstract (english)

Bacterial endotoxin content in Azithromycin injection is determined by kinetic turbidimetric method and with portable test system (PTS). Before the analysis the kinetic turbidimetric method has been validated by examining the influence of sample on inhibition/enhancement in test. The kinetic turbidimetric method is the method of choice for determination of bacterial endotoxin, but PTS method demands shorter determination time. That is why it is necessary to compare these two methods and prove that PTS method is also valid.

• Keywords: bakterijski endotoksini; kinetička turbidimetrijska metoda; prijenosni spektrofotometar
• Keywords (english): bacterial endotoxins; kinetic turbidimetric method; portable test system
• Language: croatian
• URN:NBN: urn:nbn:hr:159:030810
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-11-23 09:41:51