Sinbiotički učinak bakterija: Lactobacillus acidophilus M92, Lactobacillus plantarum L4 i Enterococcus faecium L3

doctoral thesis

• Title (english): Synbiotic effect of bacteria: Lactobacillus acidophilus M92, Lactobacillus plantarum L4 and Enterococcus faecium L3
• Author: Jadranka Frece; MBZ: 227625
• Mentor: Jagoda Šušković (mentor); MBZ: 40116
• Committee member: Zoran Zgaga (predsjednik povjerenstva); MBZ: 77902
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; Zagreb
• Defense date and country: 2007-06-18, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology
• Universal decimal classification (UDC): 60 - Biotechnology. Genetic engineering. Products of cloning

Abstract

U ovom radu istražene su mogućnosti primjene probiotičkih sojeva Lactobacillus acidophilus M92, Lactobacillus plantraum L4 i Enterococcus faecium L3 u kombinaciji s različitim prebiotičkim supstratima, u svrhu postizanja pojačanog, sinbiotičkog učinka. Asimilacija prebiotičkih supstrata s odabranim bakterijama mliječne kiseline provedena je in vitro i in vivo s pokusnim Swiss Albino miševima. Kao najbolji sinbiotički pripravci pokazali su se: L. acidophilus M92 u kombinaciji s prebiotikom inulinom, te L. plantarum L4 i E. faecium L3 u kombinaciji s laktulozom. Paralelno su provedena istraživanja imunomodulacijskog učinka ispitivanih probiotičkih sojeva i prebiotičkih supstrata na imunološki sustav miševa ELISA metodom. Adhezija ispitivanih probiotičkih sojeva u tankom i debelom crijevu pokusnih miševa potvrđena je induciranjem rezistencije ovih sojeva na rimfampicin te identifikacijom izoliranih bakterijskih sojeva iz crijeva miševa RAPD-PCR metodom.

Abstract (english)

In this research, there have been explored many possibilities of appliances probiotic strains Lactobacillus acidophilus M92, Lactobacillus plantarum L4, and Enterococcus faecium L3 in combination with various prebiotic substrates for the purpose of attaining enhanced, synbiotic effect.. The assimilation of prebiotic substrates with selected lactic acid bacteria has been realised in vitro as well as in vivo, with experimental Swiss Albino mice. The best synbiotic preparations, so far, have been: Lactobacillus acidophilus M92 in combination with prebiotic inulin, Lactobacillus plantarum L4, and Enterococcus faecium L3 in combination with lactulose. Parallel with these researches, there have been conducted immunomodulatory effect researches on tested probiotic strains and prebiotic substrates on immunity system of mice by ELISA method. Adhesive function of tested probiotic strains in the small and large intestine of experimental mice has been confirmed by inducing the resistance of these strains on rimfampicin, and by identification of isolated bacterial strains out of mice intestine by RAPD-PCR method.

• Keywords: adhezija; Enterococcus faecium; imunizacija; Lactobacillus acidophilus; Lactobacillus plantarum; prebiotik; probiotička svojstva; sinbiotik
• Keywords (english): adhesion; Enterococcus faecium; immunization; Lactobacillus acidophilus; Lactobacillus plantarum; prebiotic; probiotic properties; S-layer; synbiotic
• Language: croatian
• URN:NBN: urn:nbn:hr:159:029486
• Promotion: 2008-09-28
• Study programme: Title: Postgraduate PhD Study in Biotechnology and Bioprocess Engineering; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti (doktor/doktorica znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2018-05-14 07:41:46