Lokalizacija dijela biosintetskog puta alifatskih glukozinolata i njihova potencijalna uloga u obrani biljke

doctoral thesis

• Title (english): Localization of aliphatic glucosinolate biosynthesis and their potential role in plant defence
• Author: Ivana Radojčić Redovniković; MBZ: 266812
• Mentor: Jasna Vorkapić-Furač (mentor); MBZ: 53001
• Committee member: Vladimir Mrša (predsjednik povjerenstva); MBZ: 39525
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; Zagreb
• Defense date and country: 2007-04-05, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology
• Universal decimal classification (UDC): 60 - Biotechnology. Genetic engineering. Products of cloning

Abstract

U ovom radu istražena je stanična i sub-stanična lokalizacija metiltioalkilmalat sintaze. Ekspresije GUS proteina pokazala je promotorsku aktivnost u vaskularnom dijelu tkiva, dok sub-stanična lokalizacija nije bila moguća zbog slabog signala GPF proteina. Istražen je i utjecaj nekih bioloških i kemijskih tretmana na biosintezu glukozinolata, u inokuliranim i neinokuliranim listovima. Dobiveni rezultati upućuju na ulogu aliphatskih glukozinolata u sistemičnom odgovoru obrane biljke, budući da je utvrđena njihova povećana razine u neinokuliranim listovima.

Abstract (english)

The aim of this study was localization of methylthioalkylmalate synthase transcripts and protein in Arabidopsis thaliana. Study of GUS expression showed promoter activity exclusively in the phloem part of the vascular bundles, while sub-cellular localisation with GFP protein did not exhibit the expected results. We also studied both, local and systemic glucosinolate response, after different biological and chemical treatments. Our study points out overlooked role of aliphatic glucosinolates in plant defence especially in the systemic defence response.

• Keywords: glukozinolati; gusjenice kukaca; metal jasmonska kiselina; metiltioalkilmalat sintaza; plijesni; qRT-PCR; stanična i substanična lokalizacija
• Keywords (english): caterpillars; cellular and subcellular localisation; fungi; glucosinolates; methyl jasmonic acid; methylthioalkylmalate synthase; qRT-PCR
• Language: croatian
• URN:NBN: urn:nbn:hr:159:006511
• Promotion: 2008-09-28
• Study programme: Title: Postgraduate PhD Study in Biotechnology and Bioprocess Engineering; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti (doktor/doktorica znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2018-05-14 08:16:40