Katabolički potencijal i genetička struktura bakterijskih zajednica porijeklom iz okoliša onečišćenog atrazinom i drugim S-triazinskim spojevima

doctoral thesis

• Title (english): Catabolic potential and genetic structure of bacterial communities originating from environment contaminated with atrazine and other S-triazine compounds
• Author: Nikolina Udiković Kolić; MBZ: 245445
• Mentor: Dubravka Hršak (mentor); MBZ: 55635
• Committee member: Zoran Zgaga (predsjednik povjerenstva); MBZ: 77902
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; Zagreb
• Defense date and country: 2008-06-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology
• Universal decimal classification (UDC): 60 - Biotechnology. Genetic engineering. Products of cloning

Abstract

Osnovni cilj rada bio je izolirati i karakterizirati bakterijske zajednice iz okoliša onečišćenog atrazinom i drugim s-triazinskim spojevima. Rezultati istraživanja sa 14C obilježenim atrazinom i analiza nastalih međuproizvoda razgradnje potvrdili su da svih pet uzgojenih zajednica može mineralizirati atrazin te da katabolički put atrazina započinje deklorinacijom, nakon čega slijedi uklanjanje N-alkilnih radikala i razgradnja nastale cijanurne kiseline do CO2 i NH3. Proučavanjem genetičkog potencijala zajednica potvrđena je heterogenost i funkcionalna redundancija kataboličkih gena, najčešće smještenih na plazmidima. Analizom 16S rDNA knjižnica utvrđena je velika raznolikost i različita struktura istraživanih zajednica. Ocijenjeno je da. bi istraživane zajednice mogle poslužiti kao modelne zajednice za proučavanje biotransformacijskih procesa s-triazinskih spojeva i drugih ksenobiotika.

Abstract (english)

The objective of this work was to characterize bacterial communities originating from an environment contaminated with atrazine and other s-triazine compounds. Investigations performed with 14C-labelled atrazine and the analysis of formed intermediates showed that all five enriched communities were able to mineralize atrazine and that catabolic pathway started with dechlorination, which was followed by the removal of N-alkyl side-chains, the formation of cyanuric acid and its degradation to CO2 and NH3. The determination of atrazine-degrading genetic potential of the communities revealed hetererogeneity and functional redundancy of catabolic genes, mainly located on plasmids. The analysis of 16S rDNA clone libraries showed the high diversity as well as different structure of the studied communities. It is estimated that the studied communities can serve as model communities for investigating biotransformation processes of s-triazines and other xenobiotics.

• Keywords: atrazin; atz geni; bakterijska zajednica; biološka razgradnja atrazina; s-triazinski spojevi; trz geni
• Keywords (english): atrazine; atz genes; bacterial community; atrazine biodegradation; s-triazine compounds; trz genes
• Language: croatian
• URN:NBN: urn:nbn:hr:159:320866
• Promotion: 2009-09-13
• Study programme: Title: Postgraduate PhD Study in Biotechnology and Bioprocess Engineering; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti (doktor/doktorica znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2018-05-14 08:43:03