Biosinteza i biorazgradnja F-2 toksina i okratoksina A u tijeku rasta plijesni Fusarium graminearum ZMPBF 1244 i Aspergillus ochraceus NRRL 3174 u mješovitoj kulturi

doctoral thesis

• Title (english): Biosynthesis and biodegradation of F-2 toxin and ochratoxin A during the growth of moulds Fusarium graminearum ZMPBF 1244 and Aspergillus ochraceus NRRL 3174 in mixed culture
• Author: Domagoj Cvek; MBZ: 266845
• Mentor: Frane Delaš (mentor); MBZ: 9173
• Committee member: Ksenija Markov (predsjednik povjerenstva); MBZ: 132383
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; Zagreb
• Defense date and country: 2010-05-07, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology
• Universal decimal classification (UDC): 577 - Biochemistry. Molecular biology. Biophysics

Abstract

Cilj rada bio je istražiti utjecaj odabranih eteričnih ulja i izolata bakterija mliječne kiseline na rast plijesni Aspergillus ochraceus NRRL 3174 i Fusarium graminearum ZMPBF 1244 tijekom rasta u čistoj i mješovitoj kulturi te na biosintezu okratoksina A i F-2 toksina. Podloge za uzgoj biomase plijesni i biosintezu mikotoksina bili su kukuruzno zrnje i kvaščev ekstrakt. Rast biomase istraživanih plijesni u tekućoj podlozi određivan je gravimetrijski, a koncentracija biosintetiziranog mikotoksina ELISA metodom.

Abstract (english)

The aim of this study was to investigate the effect of selected essential oils and isolates of lactic acid bacteria on growth of the moulds Aspergillus ochraceus NRRL 3174 and Fusarium graminearum ZMPBF 1244 during the growth in pure and mixed culture and the biosynthesis of ochratoxin A and F-2 toxin. Media for growing of biomass of moulds and mycotoxin biosynthesis were corn grain, and yeast extract. The growth of biomass of studied moulds growth in liquid medium was determined gravimetrically, and the concentration of biosynthesized mycotoxin by ELISA method.

• Keywords: biosinteza; ELISA; F-2 toksin; kukuruzno zrnje; kvaščev ekstrakt; mješovita kultura; okratoksin A
• Keywords (english): biosynthesis; corn seed; ELISA; F-2 toxin; mixed culture; ochratoxin A; yeast extract
• Language: croatian
• URN:NBN: urn:nbn:hr:159:031189
• Promotion: 2010-09-12
• Study programme: Title: Biotechnology & Bioprocess Engineering; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor znanosti/doktorica znanosti (doktor znanosti/doktorica znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2018-05-15 10:16:52