Analitički aspekt odabranih ß2-adrenergičkih agonista u tkivu jetre i genotoksikološki učinci na biološkim test sustavima

doctoral thesis

• Title (english): Analytical aspect of selected ß2-adrenergic agonists in liver tissue and genotoxicity effects on biological test systems
• Author: Ana Vulić; MBZ: 292122
• Mentor: Ksenija Durgo (mentor); MBZ: 226422
• Committee member: Jasna Franekić (predsjednik povjerenstva); MBZ: 85870
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; Zagreb
• Defense date and country: 2011-12-21, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology
• Universal decimal classification (UDC): 577 - Biochemistry. Molecular biology. Biophysics

Abstract

Primjenom validirane LC-MS/MS metode određene su razine ostataka klenbuterola i salbutamola u tkivu jetre nakon subkroničnog tretmana te je potvrđena kumulacija kroz vremensko razdoblje u trajanju od 15 do 30 dana nakon završetka tretmana. Genotoksikološka ispitivanja pokazala su mutageno i prooksidativno djelovanje klenbuterola. Komet testom i analizom kromosomskih aberacija utvrđen je genotoksični učinak klenbuterola i salbutamola dok je genotoksični učinak 4-ADBA utvrđen samo komet testom.

Abstract (english)

By mean of validated LC-MS/MS method the levels of salbutamol and clenbuterol residues in liver tissue after subchronic treatment were determined and accumulation in time period of 15-30 days after treatment withdrawal was confirmed. Genotoxicological tests have shown mutagenic and prooxidative effects of clenbuterol. By mean of Comet assay and chromosome aberrations analysis genotoxic effects of clenbuterol and salbutamol were found while 4-ADBA was found genotoxic only by Comet assay.

• Keywords: ostaci klenbuterola i salbutamola; genotoksikološki učinci ß2-adrenergičkih agonista; biološki test sustavi
• Keywords (english): clenbuterol and salbutamol residues; genotoxicity of ß2-adrenergic agonists; biological test systems
• Language: croatian
• URN:NBN: urn:nbn:hr:159:205300
• Promotion: 2012-07-01
• Study programme: Title: Nutrition; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor znanosti/doktorica znanosti (doktor znanosti/doktorica znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2018-05-16 08:08:16