In vitro i in vivo modeli vezanja patulina i citrinina bioprotektivnim kulturama

doctoral thesis

• Title (english): In vitro and in vivo models of binding of mycotoxins patulin and citrinin with bioprotective cultures
• Author: Martina Bevardi
• Mentor: Ksenija Markov (mentor)
• Committee member: Frane Delaš (predsjednik povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; Zagreb
• Defense date and country: 2013-05-28, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology
• Universal decimal classification (UDC): 578/579 - Virology. Microbiology

Abstract

Cilj ovog rada bio je istražiti modele vezanja/uklanjanja patulina i citrinina bioprotektivnim kulturama bakterijom Gluconobacter oxydans 1J i komponentom stanične stijenke kvasca, β-glukanom, u in vitro i in vivo uvjetima, s ciljem moguće uporabe kao alternative postojećim metodama detoksifikacije. Određivanjem količine patulina i citrinina u uzorcima (sok i biološki materijal) uz dodatak bakterije Gluconobacter oxydans i β-glukana utvrđeno je kako u oba slučaja (in vitro i in vivo) bioprotektivne kulture imaju utjecaj na smanjenje mikotoksina. U in vitro modelu, koji prirodni jabučni sok bez dodataka aditiva, dokazano je da Gluconobacter oxydans i β-glukan, nakon 48-satne inkubacije na 25 °C, u potpunosti vežu patulin. Mikotoksin citrinin niti u jednom slučaju nije bio u potpunosti vezan, ali su obje komponente imale utjecaj na njegovo smanjenje u soku, s time da se β-glukan pokazao kao bolji agens za uklanjanje citrinina od bakterija G. oxydans. U in vivo uvjetima, gdje je pokus proveden na laboratorijskim miševima, istraživani biološki uzorci bili su feces, jetra, bubrezi, tanko i debelo crijevo, a životinje su bile imunizirane patulinom, citrininom, bakterijom Gluconobacter oxydans i β-glukanom u određenim kombinacijama. U ovom slučaju kao bolja bioprotektivna kultura pokazala se bakterija Gluconobacter oxydans i to u uzorcima fecesa, jetre i bubrega. U homogenatima tankog i debelog crijeva nisu pronađeni ostaci mikotoksina. Dobiveni rezultati upućuju na moguće modele vezanja kako patulina tako i citrinina.

Abstract (english)

The aim of this study was to examine the influence of bioprotective cultures Gluconobacter oxydans and β-glucan on reducing mycotoxin patulin and citrinin in vitro and in vivo models, with the possible use as an environmentally friendly agents. Determination of the amount of unreduced patulin and citrinin with the addition of the bacteria Gluconobacter oxydans and components of the cell wall of yeast, β-glucan, was found that in both cases (in vitro and in vivo) bioprotective cultures have an impact on the reduction of mycotoxins. In an in vitro model, which in this case was a natural apple juice with no added additives, Gluconobacter oxydans and β-glucan, after a 48 h of incubation at 25 ° C, fully bound patulin. Mycotoxin citrinin was not entirely reduced, but we proved that both components have an impact on its reduction provided that the β-glucan showed as a better decomposition agent. In in vivo models, where the experiment was conducted on mice, the biological samples we investigated were feces, liver, kidneys, small and large intestine. Animals were immunized with patulin, citrinin, Gluconobacter oxydans and β-glucan in certain combinations. In this case, a better results obtained bacteria Gluconobacter oxydans in samples of feces, liver and kidneys. In homogenates of small and large intestine were not found the remains of mycotoxins. Our results confirm how patulin and citrinin can be neutralize and / or reduced by using not toxic substances for the human body.

• Keywords: patulin; citrinin; bioprotektivne kulture; Gluconobacter oxydans; β-glukan; mikotoksini
• Keywords (english): patulin; citrinin; bioprotective cultures; Gluconobacter oxydans; β-glucan; mycotoxins
• Language: croatian
• URN:NBN: urn:nbn:hr:159:310308
• Promotion: 2013-09-22
• Study programme: Title: Postgraduate PhD Study in Biotechnology and Bioprocess Engineering; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti (doktor/doktorica znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2018-05-17 10:48:24