Raspodjela i eliminacija maduramicina u jajima nakon oralne primjene kod nesilica i razvoj LC/MS metode za određivanje maduramicina

doctoral thesis

• Title (english): Distribution and elimination of maduramicin in eggs after oral administration to laying hens and LC/MS method development for determination of maduramicin
• Author: Ivana Varenina
• Mentor: Nina Bilandžić (mentor)
• Committee member: Jagoda Šušković (predsjednik povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; Zagreb
• Defense date and country: 2015-11-06, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology
• Universal decimal classification (UDC): 636/637 - Animal husbandry and breeding. Produce of domestic animals and game

Abstract

Glavni cilj ovog rada bio je utvrditi raspodjelu i eliminaciju maduramicina u jajima nakon hranjenja kokoši nesilica krmnom smjesom s dodanim različitim masenim udjelima maduramicina, a radi usporedbe rezultata, na isti su način nesilice tretirane monenzinom i toltrazurilom. Prije određivanja rezidua, provedena je validacija metode tekućinske kromatografije s masenom spektrometrijom (LC-MS/MS) kojom je bilo obuhvaćeno 16 kokcidiostatika u jajima i 13 kokcidiostatika u krmnoj smjesi. Rezidui maduramicina su pronađeni jedino u žumanjku, dok su značajni maseni udjeli monenzina i toltrazuril-sulfona određeni i u žumanjku i u bjelanjku jajeta. Najveći maseni udio maduramicina u jajima nakon hranjenja kokoši nesilica krmnom smjesom s dodanim maduramicinom u količini od 1 % iznosa najveće dopuštene količine (NDK) tog rezidua za ciljne životinjske vrste, iznosio je 16,6 μg/kg, a nakon hranjenja s monenzinom pri istim uvjetima, u jajima je zaostalo 0,81 μg/kg monenzina. Nakon administracije toltrazurila u terapijskoj količini, najveći maseni udio toltrazuril-sulfona određen u jajima iznosio je 2741,3 μg/kg. Prema dobivenim rezultatima, u slučaju prisutnosti maduramicina u krmnoj smjesi za nesilice pri masenom udjelu koji odgovara propisanoj NDK za neciljne životinjske vrste, mogu se očekivati rezidui maduramicina u jajima iznad propisane dozvoljene količine od 12 μg/kg.

Abstract (english)

The main purpose of this study was to present deposition and elimination of maduramicin in eggs after its administration to animal food in different concentration. In order to compare results, laying hens were also treated with monensin and toltrazuril. Before determination of residues, liquid chromatography mass spectrometry (LC-MS/MS) method was developed and validated, and it covered 16 coccidiostats in eggs and 13 coccidiostats in animal food. Maduramicin residues were detected solely in egg yolk, while considerable concentration of monensin and toltrazuril-sulfone residues was detected both in egg white and egg yolk. Maximum concentration of maduramicin and monesin in whole eggs, after its administration in the animal food in amount of 1% of given maximum content (MC) for this residue in food for target species, was 16.6 μg/kg and 0,81 μg/kg, respectively. After administration of toltrazuril at therapeutic dose, maximum concentration of toltrazuril-sulfone in eggs was 2741,3 μg/kg. According to the results, maximum cross-contamination levels of maduramicin in animal food for laying hens, show residues of maduramicin in eggs higher than 12 μg/kg which is above the maximum residue limit.

• Keywords: rezidui; kokcidiostatici; jaja; LC-MS/MS; raspodjela; vrijeme zadržavanja
• Keywords (english): residues; coccidiostats; eggs; LC-MS/MS; distribution; withdrawal time
• Language: croatian
• URN:NBN: urn:nbn:hr:159:339799
• Promotion: 2016-04-17
• Study programme: Title: Postgraduate PhD Study in Biotechnology and Bioprocess Engineering; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti (doktor/doktorica znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2018-05-18 08:20:09