Praćenje učinaka ekstrakta đumbira na tumorskoj staničnoj liniji HeLa

undergraduate thesis

• Title (english): Screening of ginger extract effects on HeLa tumor cell line
• Author: Katarina Brajković
• Mentor: Višnja Gaurina Srček (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2018-06-18, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

In vitro testovima toksičnosti, može se ispitati biološka aktivnost određenih spojeva iz biljaka, a potom i procijeniti mogući učinak na ljude. Utvrđeno je da djelovanje navedenih tvari ima zaštitni učinak kod širokog raspona tumorskih oboljenja, koronarnih bolesti i srčanog udara. Stoga, sve se više ispituje primjena biološki aktivnih tvari iz biljaka kao izvora novih lijekova. U istraživanjima, koja često uključuju in vitro testove, rabe se različite humane stanične linije. U ovom radu, provedeno je in vitro ispitivanje učinka subkritičnog vodenog ekstrakta đumbira na tumorsku HeLa staničnu liniju te se pratila promjena u preživljavanju i morfologiji stanica. Rezultati su pokazali inhibitorno djelovanje ekstrakta na preživljavanje stanica, odnosno minimalnu inhibiciju od 3,99% kod tretmana s 5 mg/mL ekstrakta te maksimalnu inhibiciju od 80,96% prilikom tretmana s 50 mg/mL ekstrakta. Također, određene su i morfološke promjene u HeLa stanicama nakon djelovanja ekstrakta đumbira, koje su u skladu s rezultatima određivanja preživljavanja. Dobiveni rezultati ukazuju na potencijal primjene đumbira kao izvora biološki aktivnih spojeva.

Abstract (english)

In vitro toxicity assays can be used to investigate the biological activity of certain compounds from the plants and then evaluate the potential effect on humans. It has been established that the activities of such substances have a protective effect toward different types of tumors, coronary diseases and heart attack. Therefore, the use of biologically active substances as the source of new drugs is being tested more and more. In researches that often include in vitro tests, different human cell lines are being used. In this study, the in vitro testing of the subcritical water extract effect of ginger to human tumor cell HeLa has been carried out. The alteration in cell survival and morphology was observed. The results showed the inhibitory effect of extract on cell survival, respectively a minimum inhibition of 3.99% for 5 mg/mL extract treatment and maximum inhibition of 80.96% for 50 mg/mL extract treatment. Also, morphological changes in HeLa cells were determined after the action of ginger extract. They are consistent with survival results. The obtained results indicate the potential of ginger application as a source of biologically active compounds.

• Keywords: in vitro testovi; đumbir; HeLa stanice; vijabilnost
• Keywords (english): in vitro assays; ginger; HeLa cells; viability
• Language: croatian
• URN:NBN: urn:nbn:hr:159:037585
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-06-18 11:51:59