Nova metoda detekcije sulfita za primjenu u papirnatoj mikrofluidici

undergraduate thesis

• Title (english): New sulphite detection method for use in paper microfluidics
• Author: Valentina Čabula
• Mentor: Damir Iveković (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for General and Inorganic Chemistry and Electroanalysis); Zagreb
• Defense date and country: 2018-09-10, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U radu je po prvi puta na papirnatoj mikrofluidičkoj platformi iskušana biamperometrijska detekcija analita, pri čemu je mikrofluidička platforma posebno prilagođena mjerenju u uvjetima konstantnog protoka elektrolita. Kao modelni analit odabran je sulfit, kao relativno čest alergen, ali i prototip nizu analita koji se mogu prevesti u plinovito stanje (npr. NH₄⁺/NH₃, CO₃²⁻/HCO₃⁻/CO₂, S²⁻/HS⁻/H₂S...) i difuzijom izdvojiti iz kompleksne matrice uzorka te zbog toga vrlo selektivno detektirati i kvantificirati. Na temelju rezultata dobivenih testiranjem konstruiranog papirnatog mikrofluidičkog senzora postavljene su smjernice za daljnji razvoj i usavršavanje testirane mikrofluidičke platforme. Zaključeno je da je u daljnjem razvoju senzora potrebno riješiti probleme vezane uz promjenjivu osnovnu struju i poboljšati reproducibilnost mjerenja unaprjeđivanjem dizajna senzora i omogućavanjem provedbe mjerenja metodom standardnog dodatka.

Abstract (english)

For the first time, biamperometric analytical detection was tested in the paper microfluidic platform, which is specifically adapted for measurement under conditions of constant electrolyte flow. As a model analyte are selected sulphides, as a relatively common allegen, but also as a prototype series of volatile analytes that can be converted into gas (ex. NH₄⁺/NH₃, CO₃²⁻/HCO₃⁻/CO₂, S²⁻/HS⁻/H₂S...), diffuse and separate from the complex sample matrix and therefore highly selectively be detected and quantified. Based on the results obtained by testing the constructed paper microfluidic sensor, guidelines for further development and improvement of the tested microfluidic platform were laid down. It was concluded that in the further development of the sensor, problems related to variable primary current need to be solved and reproducibility of measurements improved by enhancing the design of the sensor and enabling the measurement to be carried out using the standard plug-in method.

• Keywords: mikrofluidika na papiru; plinska difuzija; senzor; sulfit
• Keywords (english): gas diffusion; paper-based microfluidic; sensor; sulphite
• Language: croatian
• URN:NBN: urn:nbn:hr:159:098418
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-09-12 08:32:29