Biološki učinak proteinskih hidrolizata iz pogače konoplje na tumorsku staničnu liniju MCF-7

undergraduate thesis

• Title (english): The biological effect of hemp seed protein hydrolysates on MCF-7 tumor cell line
• Author: Jelena Kosanović
• Mentor: Višnja Gaurina Srček (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2018-09-19, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Bioaktivni peptidi posjeduju širok raspon bioloških svojstava koja mogu utjecati na poboljšanje ljudskog zdravlja. Zbog toga se smatraju novom generacijom biološki aktivnih regulatora, koji pronalaze potencijalnu primjenu kao sastojci nutraceutika, te farmaceutika. Cilj ovog rada bio je ispitati učinak proteinskog hidrolizata konoplje, kao i njegove frakcije <3 kDa, na rast i proliferaciju tumorske stanične linije MCF-7. Stanice su tretirane različitim koncentracijama proteinskih hidrolizata konoplje (1, 2 i 5 mg/mL), nakon čega je određena vijabilnost stanica (MTS). Rezultati su pokazali da proteinski hidrolizat konoplje, pri koncentracijama 2 i 5 mg/mL, ima inhibitorni učinak na preživljenje stanica i to u rasponu od 53,96% do 62,17%. S druge strane, frakcija proteinskog hidrolizata konoplje <3 kDa je pri maksimalnoj ispitivanoj koncentraciji (5 mg/mL) djelovala blago stimulativno (10,07%) na preživljenje stanica, dok pri nižim koncentracijama nije zapažen nikakav učinak. Za dobivanje kompletne slike o djelovanju proteinskih hidrolizata konoplje i njihovih frakcija, potrebno je provesti dodatna istraživanja, kako na MCF-7 stanicama, tako i na drugim staničnim linijama.

Abstract (english)

Bioactive peptides have a wide range of biological properties that may affect the improvement of human health. Therefore, they are considered the new generation of biologically active regulators that find their potential use as nutraceuticals and pharmaceuticals ingredients. The aim of this study was to examine the effect of hemp seed protein hydrolysate as well as its peptide fraction <3 kDa on growth and proliferation of the tumor cell line MCF-7. MCF-7 cells were treated with different concentrations of hemp seed protein hydrolysates (1, 2 and 5 mg/mL), and then cell viability was determined (MTS). The results showed that hemp seed protein hydrolysate of 2 and 5 mg/mL had an inhibitory effect on cell viability (53,96% to 62,17%). On the other hand, hemp seed protein hydrolysate fraction <3 kDa has shown slight stimulatory effect (10,07%) on cell viability at maximal examinated concentration (5 mg/mL), while at lower concentrations no effect was shown. To obtain a wider picture of the action of hemp seed protein hydrolysates and their fractions, further research is needed, both on MCF-7 cells and other cell lines.

• Keywords: bioaktivni peptidi; proteinski hidrolizati konoplje; MCF-7 stanična linija; vijabilnost
• Keywords (english): bioactive peptides; hemp seed protein hydrolysates; MCF-7 cell line; viability
• Language: croatian
• URN:NBN: urn:nbn:hr:159:331045
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-09-24 07:49:21