β-glukan kao biofiksator aflatoksina M1

undergraduate thesis

• Title (english): β-glucan as a biofixator of aflatoxin M1
• Author: Marko Baruškin
• Mentor: Jadranka Frece (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for General Microbiology and Food Microbiology); Zagreb
• Defense date and country: 2018-09-20, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Metode uklanjanja i/ili redukcije mikotoksina koje se koriste u prehrambenoj industriji troše velike količine energije i kemijskih sredstava, stoga biološke metode sve više dobivaju na značaju zbog svoje netoksičnosti i ekološkog aspekta. Cilj ovog rada je bio ispitati djelotvornost komercijalnog β-glukana dobivenog iz zobi i β-glukana izoliranog iz kvaščeve biomase prilikom vezanja aflatoksina M1 iz umjetno kontaminiranog mlijeka. Obje vrste β-glukana su pokazale sposobnost vezanja AFM1, iako je β-glukan dobiven iz zobi pokazao malo veću efikasnost vezanja (65%) u odnosu na β-glukan izoliran iz kvasca (63,6%). Također je uočljiva nelinearnost prilikom vezanja toksina tijekom 24 sata β-glukanom iz kvasca u usporedbi s β-glukanom iz zobi, što se može objasniti reverzibilnom reakcijom stvaranja kompleksa AFM1- β-glukan.

Abstract (english)

The methods of removing and/or reducing mycotoxins used in food industry consume large amounts of energy and chemical resources, so biological methods are becoming increasingly important due to their non-toxicity and ecological aspects. The aim of this thesis was to investigate the efficacy of commercial β-glucan obtained from oats and β-glucan isolated from yeast biomass on binding aflatoxin M1 from artificially contaminated milk. Both types of β-glucans demonstrated the ability to bind AFM1, although β-glucan obtained from the oats showed slightly higher binding efficacy (65%) than β-glucan isolated from yeast (63,6%). There is also notable nonlinearity during 24 hours of toxin binding with β-glucan from yeast compared to β-glucan from oats, which can be explained by the reversible reaction of AFM1-β-glucan complex formation.

• Keywords: aflatoksin M1; biološke metode; mikotoksini; β-glukan
• Keywords (english): aflatoxin M1; biological methods; mycotoxins; β-glucan
• Language: croatian
• URN:NBN: urn:nbn:hr:159:791896
• Study programme: Title: Food Technology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-10-04 09:05:11