Preživljavanje i morfologija tumorske stanične linije MCF-7 u prisustvu ekstrakata đumbira

undergraduate thesis

• Title (english): The viability and morphology of tumor MCF-7 cells in the presence of ginger extracts
• Author: Željka Stanečić
• Mentor: Višnja Gaurina Srček (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2018-09-19, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U suvremenom svijetu primjena biološki aktivnih tvari iz biljaka koje imaju blagotvoran učinak na organizam sve je raširenija. Za određivanje biološke aktivnosti spojeva iz prirodnih izvora često se koriste in vitro testovi koji uključuju uporabu različitih humanih staničnih linija. Pomoću in vitro testova najčešće se ispituje tzv. bazalna citotoksičnost kojom se određuje učinak ispitivane tvari na preživljavanje stanica u kulturi. U ovom radu provedeno je in vitro ispitivanje učinaka subkritičnih vodenih ekstrakata đumbira na tumorskoj staničnoj liniji MCF-7 te je određena vijabilnost stanica MTS metodom 48 sati nakon tretmana. Rezultati su pokazali inhibitorno djelovanje ekstrakta na preživljavanje stanica, a maksimalna inhibicija od 61,21% postignuta je tretiranjem stanica sa 100 mg/mL ekstrakta. Morfološke promjene u stanicama nakon tretmana praćene su primjenom svjetlosne i fluorescentne mikroskopije te su u korelaciji s rezultatima određivanja vijabilnosti stanica. Rezultati dobiveni u ovom istraživanju ukazuju na mogućnost primjene đumbira kao izvora biološki aktivnih spojeva.

Abstract (english)

Nowadays, use of biologically active substances from plant sources with beneficiary effects on human health is increasing. Biological potential of such substances is often studied by in vitro tests involving the use of human cell lines. In vitro tests are usually assessed basal cytotoxicity by determining the effects of the test substance on cell culture survival. In this study effects of subcritical water extract of ginger has been studied in vitro on human tumor cell line MCF-7. Cell viability was determined by MTS method after 48 hours of treatment. The results showed the inhibitory effect of extracts on cell survival, and maximum inhibition of 61,21% was achieved by treatment of cells with 100 mg/mL of extract. Morphological changes in cells after treatment are determined by light and fluorescence microscopy and correlated to the viability results. The obtained results of this study indicate the possibility of using ginger as a source of biologically active compounds.

• Keywords: đumbir; ekstrakt; in vitro testovi; MCF-7 stanice; vijabilnost
• Keywords (english): extract; ginger; in vitro assays; MCF-7 cells; viability
• Language: croatian
• URN:NBN: urn:nbn:hr:159:025799
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-10-10 09:14:03