Pojačana ekspresija alela YAP1C620F povećava otpornost kvasca na octenu kiselinu i 2-furaldehid

undergraduate thesis

• Title (english): Overexpression of the YAP1C620F allele increases resistance of the yeast to acetic acid and 2-furaldehyde
• Author: Nina Leitner
• Mentor: Anamarija Štafa (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2019-06-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Svjetske zalihe fosilnih goriva se smanjuju, a zbog onečišćenja okoliša uzrokovanog njihovom prekomjernom upotrebom potrebno je naći ekonomičniju i održiviju alternativu. Proizvodnja bioetanola pomoću kvasca Saccharomyces cerevisiae, koristeći obnovljivi izvor, lignocelulozne sirovine, jedna je od alternativa za proizvodnju goriva. Kvasac ne može direktno fermentirati lignoceluozne sirovine te ih je stoga potrebno prvo hidrolizirati kako bi se oslobodili fermentabilini šećeri, ali predtretmanom lignoceluloznih sirovina nastaju i inhibitori koji usporavaju ili onemogućuju rast mikroorganizama te se stoga mnoga istraživanja temelje na konstrukciji proizvodnih sojeva otpornih na inhibitore rasta. U ovome radu transformiran je soj kvasca Žup ura- plazmidom pSP-YAP1-C620F koji omogućuje pojačanu ekspresiju konstitutivno aktivnog alelaYAP1C620F, za kojeg je pokazano da povećava otpornost nekih sojeva kvasca S. cerevisiae na inhibitore rasta. Konstruirani soj Žup YAP1C620F pokazao je veću otpornost na octenu kiselinu i 2-furaldehid u usporedbi sa sojem koji ima pojačanu ekspresiju divljeg tipa gena YAP1.

Abstract (english)

World supplies of fossil fuels are decreasing and, due to the environmental pollution caused by their excessive use, it is necessary to find a more economical and a more sustainable alternative. Fermentation of renewable lignocellulose substrates to ethanol by the yeast Saccharomyces cerevisiae is one of the alternatives for fuel production. Since yeast cannot directly ferment lignocellulose substrates, it is first necessary to hydrolase lignocellulose in order to release fermentable sugars. Moreover, during the pretreatment of lignocellulose biomass, different inhibitors that slow down or inhibit growth of microorganisms are formed. Therefore, various attempts have been made to construct a production strain that will be resistant to those growth inhibitors. In this research, the yeast strain Žup ura- was transformed with the pSP-YAP1-C620F plasmid that enables overexpression of constitutively active gene YAP1C620F that, for some strains of the yeast S. cerevisiae, increases resistance to growth inhibitors. Constructed strain with overexpression of YAP1C620F allele indeed displayed increased resistance to acetic acid and 2-furaldehyde in comparison to a strain that has overexpression of the wild type YAP1 gene.

• Keywords: kvasac; transformacija; gen YAP1; lignocelulozne sirovine; inhibitori rasta
• Keywords (english): yeast; transformation; YAP1 gene; lignocellulosic substrates; growth inhibitors
• Language: croatian
• URN:NBN: urn:nbn:hr:159:299267
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2019-07-12 09:03:02