Primjena eutektičkih otapala u valorizaciji otpada narančine kore

undergraduate thesis

• Title (english): Application of deep eutectic solvents in valorisation of orange peel waste
• Author: Ana Novak
• Mentor: Marina Cvjetko Bubalo (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2019-09-09, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Koncept valorizacije otpada nastalog preradom citrusa koji uključuje i narančinu koru predstavlja potencijalno rješenje za smanjenje onečišćenja okoliša te dodatno iskorištenje otpada. Kako bi se proveo postupak izdvajanja bioloških spojeva kore naranče s dodanom vrijednošću, prema ekološki prihvatljivim načelima poželjno je korištenje zelenih otapala kao što su eutektička otapala. Stoga, u ovom je radu ispitana mogućnost primjene prirodnog eutektičkog otapala kolin-klorid:glicerola sa 30, 50 i 80 % vode (w/w) u valorizaciji otpadne kore naranče. Ispitano eutektičko otapalo, u usporedbi s konvencionalnim otapalima, pokazalo se pogodnim za ekstrakciju polifenolnih spojeva , a nepovoljnim za ekstrakciju proteina. Također je ispitana aktivnost enzima pektin-esteraze ekstrahiranog iz otpadne narančine kore, pri čemu je pektin-esteraza pokazala značajno veću aktivnost u eutektičkom otapalu u odnosu na pufer. Osim toga, otapalo kolin-klorid:glicerol se pokazalo boljim otapalom za enantioselektivnu hidrolizu (R,S)-1-feniletil-acetata primjenom hidrolitičkih enzima iz narančine kore u odnosu na pufer.

Abstract (english)

The concept of waste valorisation comes from the processing of citrus, which includes the orange peel, as a potential solution to reduce environmental pollution and benefit from waste utilization. In order to make the valorisation of orange peel waste concept fully based on environmentally acceptable principles,during the process of extracting biological active compounds, green solvents such as deep eutectic solvents (DESs) are desirable. In this paper, the possibility of using choline chloride:glycerol with 30%, 50% and 80% of water (w/w) in the valorisation of waste orange peel has been investigated. Investigated DES was found to be a good substitute for acidified ethanol in extraction of polyphenols from orange peel, however, it showed low protein extraction efficiency. The activity of pectin-esterase, which was extracted from waste orange peel, was also examined and it was observed that pectin-esterase exhibit significantly higher activity in DES in comparison to the buffer. Furthermore, choline chloride:glycerol was shown to be more efficient solvent for the enantioselective hydrolysis of (R,S)-1-phenylethyl acetate during the usage of hydrolytic enzymes from orange peel in comparison to buffer.

• Keywords: eutektičko otapalo; kora naranče; pektin-esteraza; polifenoli; (R; S)-1- feniletil-acetat; valorizacija organskog otpada
• Keywords (english): deep eutectic solvent; orange peel; pectin-esterase; polyphenols; (R; S)-1- phenylethyl acetate; valorisation of organic waste
• Language: croatian
• URN:NBN: urn:nbn:hr:159:449278
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-09-09 12:51:25