Karakterizacija animalnog modela tauopatije odabranim testovima ponašanja

undergraduate thesis

• Title (english): Characterization of the animal model of tauopathy by using selected behavioral tests
• Author: Emma Štampar
• Mentor: Blaženka Kos (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology); Zagreb
• Defense date and country: 2019-09-09, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Sporadična Alzheimerova bolest (AB) najčešća je sekundarna tauopatija. Istraživanja na animalnim modelima AB pokazala su transsinaptičko širenje monomernog, oligomernog i fibrilarnog oblika tau proteina, međutim nije jasno utvrđeno je li takvo širenje popraćeno i predmnijevanim kognitivnim promjenama. Stoga je cilj ovog rada bio odrediti hoće li širenje određenog oblika tau proteina biti povezano s kognitivnim promjenama. Nakon 3 i 6 mjeseci po injiciranju tau proteina u entorinalnu moždanu koru Wistar štakora provedeni su odabrani testovi ponašanja. Rezultati dobiveni nakon provedenih testova ponašanja su pokazali da je kognitivni deficit bio ovisan o obliku injiciranog tau proteina i vremenskoj točci u kojoj je izvršeno testiranje. Tri mjeseca nakon injiciranja, testom otvorenog prostora utvrđeno je anksiozno ponašanje kod skupina životinja kojima su injicirani oligomeri tau proteina i skupina životinja kojima su injicirani kratki filamenti tau proteina. T-labirintom je utvrđen deficit kratkoročnog pamćenja kod skupine fibrili nakon 3 i 6 mj i kod skupine oligomer nakon 6 mj, dok testom prepoznavanja novog objekta nije utvrđen deficit dugoročnog pamćenja ni u jednoj od ispitivanih skupina.

Abstract (english)

Sporadic Alzheimer's Disease (AD) is the most common secondary tauopathy. Research on animal models of AB has shown the trans-synaptic spread of the monomeric, oligomeric, and fibrillar forms of tau proteins. However, it has not been clearly determined whether such propagation parallels presumed cognitive changes. Therefore, the aim of this work was to determine will the spread of tau proteins be accompanied with cognitive changes. Three and six months after injection of tau protein into the entorhinal cortex, selected behavioral tests were performed. Behavior tests results showed that rats exhibited form and time-dependent cognitive deficits. The open field test showed anxiety behavior in the groups receiving the tau oligomer and the groups receiving the tau fibril at 3 m time point. The T-maze test showed a short-term memory deficit in the fibrils group after both 3 and 6 m, and in the oligomer group after 6 m. The novel object recognition test showed no long-term memory deficit in either group.

• Keywords: Alzheimerova bolest; animalni model; memorija; tau protein; testovi ponašanja
• Keywords (english): Alzheimer's disease; animal model; behavioral tests; memory; tau protein
• Language: croatian
• URN:NBN: urn:nbn:hr:159:610281
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2019-09-11 11:20:24