Predobrada jelove piljevine razrijeđenom sumpornom kiselinom u visokotlačnom reaktoru

undergraduate thesis

• Title (english): Dilute acid pretreatment of fir sawdust in a high-pressure reactor
• Author: Zora Kelemen
• Mentor: Vlatka Petravić Tominac (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2019-09-18, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Kao lignocelulozna sirovina u ovom radu je korištena jelova piljevina te je tretirana razrijeđenom sumpornom kiselinom (0.5% w/v) u visokotlačnom reaktoru pri različitim temperaturama (180°C, 200°C i 210°C) i vremenima zadržavanja (5, 10 i 15 minuta). Prikladnost pojedinih uvjeta predobrade procijenjena je obzirom na koncentracije šećera i inhibitora u dobivenim kiselinskim hidrolizatima. Najbolji sastav imao je hidrolizat dobiven pri temperaturi od 180°C uz vrijeme zadržavanja 5 minuta. Pritom je sastav hidrolizata bio slijedeći: glukoza (2.08g/L), manoza s primjesama ksiloze i galaktoze (7.17g/L), arabinoza (0.68g/L), topljivi lignin (0.20g/L), octena kiselina (6.86g/L), levulinska kiselina (0.03g/L) i furani (0.24g/L) dok mravlja kiselina nije detektirana. U svim primijenjenim uvjetima predobrade došlo je do razgradnje celuloze i jedini polisaharid detektiran u čvrstoj fazi bio je glukan. Kako bi se izbjegla prekomjerna razgradnja celuloze i nastajanje inhibitora, potrebno je provesti optimiranje predobrade primjenom blažih uvjeta hidrolize.

Abstract (english)

In this theisis, fir sawdust was hydrolysed using dilute sulphuric acid (0.5%w/v) pretreatment in a high-pressure reactor at three different temperatures (180°C, 200°C i 210°C) and three residence times (5, 10 i 15 minutes). Adequacy of conditions was approximated with regards to sugar and inhibitor concentrations in the hydrolysates. The best results were recovered during a 5 minute pretreatment at 180°C. The composition of the obtained hydrolysates was as following: glucose (2.08g/L), manose with xylose and galactose admixture (7.17g/L), arabinose (0.68g/L), soluble lignin (0.20g/L), acetic acid (6.86g/L), levulinic acid (0.03g/L), furans (0.24g/L) while formic acid was undetected. In all pretreatment conditions cellulose was hydrolysed and the only polisaccharide detected in solid phase was glucan. To avoid redundant disintegration of cellulose and forming of inhibitors, the pretreatment should be optimized by applying milder conditions of hydrolysis.

• Keywords: lignoceluloza; jelova piljevina; kiselinska predobrada; meko drvo
• Keywords (english): lignocellulose; fir sawdust; acid pretreatment; softwoods
• Language: croatian
• URN:NBN: urn:nbn:hr:159:938001
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2019-09-23 11:42:52