Djelovanje ekstrakata iz otpada proizvodnje kakaa na in vitro rast stanica i stanični ciklus

undergraduate thesis

• Title (english): Effect of extracts from cocoa waste on in vitro cell growth and cell cycle
• Author: Martina Kolak
• Mentor: Kristina Radošević (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2019-09-20, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Nizom istraživanja pokazano je da otpad nastao nakon proizvodnje kakaa sadrži visok udio polifenolnih komponenata koje imaju značajan antioksidacijski kapacitet te sveukupno pozitivno djelovanje na zdravlje čovjeka. Obzirom na potrebu učinkovitog zbrinjavanja otpada iz industrije te moguće dodatne valorizacije sve češće se nastali otpad dodatno iskorištava kako bi se proizveli novi visokovrijedni proizvodi. Budući da se industrija okreće zelenim, održivim tehnologijama, izolacija polifenola iz otpada trebala bi biti bazirana na alternativnim metodama ekstrakcije. Stoga je u ovom radu proučavano djelovanje ekstrakata koji su pripravljeni pomoću prirodnih eutektičnih otapala (NADES) koji zadovoljavaju principe zelene kemije. Biološka aktivnost pripremljenih ekstrakata ispitana je u in vitro uvjetima na „normalnoj“ humanoj staničnoj liniji HaCaT i tumorskoj humanoj staničnoj liniji HeLa. Ispitano je preživljenja stanica te utjecaj na stanični ciklus 48h nakon tretmana pripravljenim ekstraktima. Zaključno, ekstrakti su pokazali inhibitorno djelovanje na HeLa tumorsku staničnu liniju dok su na rast normalne HaCaT stanične linije djelovali pozitivno. Zapaženi učinak na rast stanica u korelaciji je sa staničnim ciklusom.

Abstract (english)

A series of studies have shown that waste, produced after cocoa production, contains a high proportion of polyphenolic components that have significant antioxidant capacity and an overall positive effect on human health. Due to the need for efficient disposal of waste from industry and possible additional valorisation, the collected waste is increasingly exploited in order to produce new high value products. As the industry turns to green, sustainable technologies, the isolation of polyphenols from waste should be based on alternative extraction methods. Therefore, the action of extracts prepared using natural eutectic solvents (NADES) that satisfy the principles of green chemistry is studied in this paper. The biological activity of the prepared extracts was examined under in vitro conditions on a "normal" human HaCaT cell line and a tumor human HeLa cell line. Cell viability and the effect on the cell cycle 48h after treatment with the extracts were examined. In conclusion, the extracts showed an inhibitory effect on the HeLa tumor cell line, while they had a positive effect on the growth of normal HaCaT cell line. The observed effect on cell growth is correlated with the cell cycle.

• Keywords: otpad od proizvodnje kakaa; antioksidacijski kapacitet; stanični ciklus; stanične linije; protočna citometrija
• Keywords (english): waste from cocoa production; antioxidant potential; cell cycle; cell lines; flow cytometry
• Language: croatian
• URN:NBN: urn:nbn:hr:159:237243
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2019-09-24 08:35:25